Journal of the Brazilian Chemical Society
Print version ISSN 0103-5053
MATA, Rosalina C. S. et al. Molluscicidal Activity of Compounds Isolated from Euphorbia conspicua N. E. Br. J. Braz. Chem. Soc. [online]. 2011, vol.22, n.10, pp. 1880-1887. ISSN 0103-5053. http://dx.doi.org/10.1590/S0103-50532011001000008.
Euphorbia conspicua latex was fractionated into triterpenic and irritant fractions I and II. The triterpenic fraction afforded 15 known compounds and a new triterpene, 3β-(E)-cinnamoyleuphorbol. 20-O-Acetyl-3-O-angeloylingenol was isolated from irritant fraction II. The compounds euphol, 3β-acetoxyeupha-8,24-diene, 3β-(E)-cinnamoyleuphorbol and 20-O-Acetyl-3-O-angeloylingenol were evaluated for molluscicidal activity. 20-O-Acetyl-3-O-angeloylingenol presented LC100 value of 1 mg mL-1, equivalent to that of the standard molluscicide niclosamide. Compounds euphol, 3β-acetoxyeupha-8,24-diene and 3β-(E)-cinnamoyleuphorbol showed low molluscicidal activity. Mutagenic assays (Ames test with strains TA 98, 100 and 102) were performed with 3β-(E)-cinnamoyleuphorbol in the presence and absence of metabolic activation (S9 mix). In V79 cells, the cytotoxicity of 3β-(E)-cinnamoyleuphorbol was evaluated using the MTT assay and the genotoxicity was assessed using the cytokinesis-block micronucleus assay (CBMN) with or without S9 mix. Mutagenic or genotoxic activity was not detected, and no significant cytotoxicity was observed for 3β-(E)-cinnamoyleuphorbol at lower doses.
Keywords : Euphorbia conspicua; molluscicidal activity; mutagenic activity; cytotoxic activity; genotoxicity; 3β-(E)-cinnamoyleuphorbol; 20-O-acetyl-3-O-angeloylingenol.