SciELO - Scientific Electronic Library Online

vol.23 issue8Lanthanide nitrates as Lewis acids in the one-pot synthesis of 1,2,4-oxadiazole derivativesKinetic analytical method for determination of uric acid in human urine using analyte pulse perturbation technique author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Journal of the Brazilian Chemical Society

Print version ISSN 0103-5053


CHARAO, Mariele F. et al. Simultaneous quantification of lycopene, β-carotene, retinol and α -tocopherol in plasma after a simple extraction procedure: stability study and application to human volunteers. J. Braz. Chem. Soc. [online]. 2012, vol.23, n.8, pp.1441-1449.  Epub July 31, 2012. ISSN 0103-5053.

A method for the simultaneous quantification of lycopene, β-carotene, retinol and α-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20°C for three months for stability study. The methodology was applied to samples from painters and individuals not exposed to paints (n = 75). The assay was linear for all vitamins (r > 0.99). Intra- and inter-run precisions were obtained with coefficient of variation smaller than 5%. The accuracies ranged from 0.29 to -5.80% and recoveries between 92.73 and 101.97%. Plasma samples and extracted supernatants were stable for 60 days at -20°C. A significant decrease of lycopene, β-carotene and retinol concentrations in plasma from exposed individuals compared to non-exposed individuals (p < 0.05) was observed. The method is simple, reproducible, precise, accurate and sensitive, and can be routinely utilized in clinical laboratories.

Keywords : retinol; α-tocopherol; lycopene; β-carotene; HPLC; liquid-liquid extraction.

        · abstract in Portuguese     · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License