Abstract

ULIANA, Carolina V.; GARBELLINI, Gustavo S.  and  YAMANAKA, Hideko. Spectrophotometric evaluation of the behavior of disperse red 1 dye in aqueous media and its interaction with calf thymus ds-DNA. J. Braz. Chem. Soc. [online]. 2012, vol.23, n.8, pp. 1469-1475.  Epub Aug 07, 2012. ISSN 0103-5053.  http://dx.doi.org/10.1590/S0103-50532012005000009.

UV-Vis spectrophotometry was used to measure the solubility of disperse red 1 (DR1) dye in aqueous solutions, using different solvents and dispersants. The dye was quantified in water samples, and its interaction with calf thymus ds-DNA was investigated. The results showed that the commercial dispersant Fongranal^® FB was suitable for the preparation of aqueous DR1 solutions. For the determination of DR1 in purified water, the limit of detection was 2.47 × 10^-6 mol L^-1, and the limit of quantification was 8.22 × 10^-6 mol L^-1. Percentage recoveries of 91.2 and 103% were obtained for DR1 concentrations of 3.00 × 10^-6 and 40.0 × 10^-6 mol L^-1, respectively. The recoveries achieved for DR1 present in tap and river water were in the range 85.9-113%. The interaction of DR1 with calf thymus DNA was accompanied by hypochromic and hyperchromic effects, which were related to conformational changes and damage to the DNA double helix.

Keywords : disperse red 1; textile dye; solubility; ds-DNA.