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vol.20 issue1Shelf-life of a 2.5% sodium hypochlorite solution as determined by arrhenius equationEffect of APF gel application time on enamel demineralization and fluoride uptake in situ author indexsubject indexarticles search
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Brazilian Dental Journal

Print version ISSN 0103-6440On-line version ISSN 1806-4760


LANA, Patricia Elaine Panicali et al. Antimicrobial activity of calcium hydroxide pastes on Enterococcus faecalis cultivated in Root Canal Systems. Braz. Dent. J. [online]. 2009, vol.20, n.1, pp.32-36. ISSN 0103-6440.

The effectiveness of calcium hydroxide pastes: Calen™ and PMCC-Calen™ associated to chemo-mechanical preparation was assessed on Enterococcus faecalis grown within root canals. Seventy incisors were inserted into TSB medium, sterilized and contaminated with E. faecalis. Culture medium was replaced each 24 h and incubated at 37oC for 72 h. After chemo-mechanical preparation, root canals were filled with Calen™ or PMCC-Calen™ (7 or 14 days). Pastes were removed and teeth were inserted into test tubes containing Enterococcosel broth. Calen™ paste (maintained for 7 and 14 days) induced 70% elimination of enterococci and PMCC-Calen™ 100% elimination only after maintenance for 14 days. These medications were significantly more effective (p<0.001) than chemo-mechanical protocol alone and PMCC-Calen™ maintained for 7 days, both incapable to eliminate the viability of enterococci. Calcium hydroxide pastes demonstrated important adjuvant effects in the elimination of enterococci during chemo-mechanical preparation of root canal systems. When associated with PMCC, calcium hydroxide pastes should be maintained for at least 14 days.

Keywords : calcium hydroxide; chemo-mechanical preparation; Enterococcus faecalis.

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