Stute et al. 2121 Stute, N; Holtz, K; Bubenheim, M; Lange, C; Blake, F; Zander, AR. Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use. Exp Hematol 2004;32:1212-1225.
|
1%, 3%, and 10% HS |
10% FBS |
BMSC |
Doucet et al. 6262 Doucet, C; Ernou, I; Zhang, Y; Llense, JR; Begot, L; Holy, X; et al.. Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications. J Cell Physiol 2005;205:228-236.
|
5% HPL |
10% FBS |
BMSC |
Shahdadfar et al. 8080 Shahdadfar, A; Fronsdal, K; Haug, T; Reinholt, FP; Brinchmann, JE. In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability. Stem Cells 2005;23:1357-1366.
|
20% HS |
FBS 20% |
BMSC |
Gregory et al. 8181 Gregory, CA; Reyes, E; Whitney, MJ; Spees, JL. Enhanced engraftment of mesenchymal stem cells in a cutaneous wound model by culture in allogenic species-specific serum and administration in fibrin constructs. Stem Cells 2006;24:2232-2243.
|
20% HS and 10% HS + grow factors |
FBS 20% |
BMSC |
Muller et al. 9595 Muller, I; Kordowich, S; Holzwarth, C; Spano, C; Isensee, G; Staiber, A; et al.. Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. Cytotherapy2006;8:437-444.
|
2.5% and 5% HPL |
10% FBS |
BMSC |
Vogel et al. 111111 Vogel, JP; Szalay, K; Geiger, F; Kramer, M; Richter, W; Kasten, P. Platelet-rich plasma improves expansion of human mesenchymal stem cells and retains differentiation capacity and in vivo bone formation in calcium phosphate ceramics. Platelets2006;17:462-469.
|
3% PRP |
FBS 2% |
BMSC |
Le Blanc et al. 8282 Le Blanc, K; Samuelsson ,H; Lonnies, L; Sundin, M; Ringden, O. Generation of immunosuppressive mesenchymal stem cells in allogeneic human serum. Transplantation2007;84:1055-1059.
|
10% HS |
10% FBS |
BMSC |
Capelli et al. 6060 Capelli, C; Domenghini, M; Borleri, G; Bellavita, P; Poma, R; Carobbio, A; et al.. Human platelet lysate allows expansion and clinical grade production of mesenchymal stromal cells from small samples of bone marrow aspirates or marrow filter washouts. Bone Marrow Transplant 2007;40:785-791.
|
5% HPL |
FxBS 10% |
BMSC |
Kocaoemer et al. 8989 Kocaoemer, A; Kern, S; Kluter, H; Bieback, K. Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. Stem Cells 2007;25:1270-1278.
|
10% HS and 10% aPRP |
10% FBS |
ASC |
Lange et al. 7878 Lange, C; Cakiroglu, F; Spiess, AN; Cappallo-Obermann, H; Dierlamm, J; Zander, AR. Accelerated and safe expansion of human mesenchymal stromal cells in animal serum-free medium for transplantation and regenerative medicine. J Cell Physiol 2007;213:18-26.
|
5% HPL |
10% FBS |
BMSC |
Lataillade et al. 102102 Lataillade, JJ; Doucet, C; Bey, E; Carsin, H; Huet, C; Clairand, I; et al.. New approach to radiation burn treatment by dosimetry-guided surgery combined with autologous mesenchymal stem cell therapy. Regen Med 2007;2:785-794. * |
8% HPL |
- |
BMSC |
Reinisch et al. 7272 Reinisch, A; Bartmann, C; Rohde, E; Schallmoser, K; Bjelic-Radisic, V; Lanzer, G; et al.. Humanized system to propagate cord blood-derived multipotent mesenchymal stromal cells for clinical application. Regen Med2007;2:371-382.
|
10% HPL |
10% FBS |
UCMSC and BMSC |
Schallmoser et al. 7575 Schallmoser, K, Bartmann, C, Rohde,E, Reinisch, A, Kashofer, K, Stadelmeyer, E, et al.. Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells. Transfusion2007;47:1436-1446.
|
10% HPL |
10% FBS |
BMSC |
Schallmoser et al. 114114 Schallmoser, K; Rohde, E; Reinisch, A; Bartmann, C; Thaler, D; Drexler, C; et al.. Rapid large-scale expansion of functional mesenchymal stem cells from unmanipulated bone marrow without animal serum. Tissue Eng Part C Methods 2008;14:185-196.
|
5% and 10% HPL |
10% FBS |
BMSC |
Shayesteh et al. 9797 Shayesteh, YS; Khojasteh, A; Soleimani, M; Alikhasi, M; Khoshzaban, A; Ahmadbeigi, N. Sinus augmentation using human mesenchymal stem cells loaded into a beta-tricalcium phosphate/hydroxyapatite scaffold. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;106:203-209. * |
20% HS |
- |
BMSC |
Zaky et al. 7070 Zaky, SH; Ottonello, A; Strada, P; Cancedda, R; Mastrogiacomo, M. Platelet lysate favours in vitro expansion of human bone marrow stromal cells for bone and cartilage engineering. J Tissue Eng Regen Med 2008;2:472-481.
|
5% HPL and 5% HPL + 10% FBS |
10% FBS and FGF2 |
BMSC |
Behnia et al. 9999 Behnia, H; Khojasteh, A; Soleimani, M; Tehranchi, A; Khoshzaban, A; Keshel, SH; et al.. Secondary repair of alveolar clefts using human mesenchymal stem cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:e1-6.
|
20% HS |
- |
BMSC |
Bieback et al. 2929 Bieback, K; Hecker, A; Kocaomer, A; Lannert, H; Schallmoser, K; Strunk, D; et al.. Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow. Stem Cells 2009;27:2331-2341.
|
10% aPRP and 10% HPL and 10% HS |
10% FBS |
BMSC |
Blande et al. 6464 Blande, IS; Bassaneze, V; Lavini-Ramos, C; Fae, KC; Kalil, J; Miyakawa, AA; et al.. Adipose tissue mesenchymal stem cell expansion in animal serum-free medium supplemented with autologous human platelet lysate. Transfusion2009;49:2680-2685.
|
2.5% or 10% HPL |
10% FBS |
ASC |
Lindroos et al. 8686 Lindroos, B; Boucher, S; Chase, L; Kuokkanen, H; Huhtala, H; Haataja, R; et al.. Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro. Cytotherapy2009;11:958-972.
|
10% HS |
10% FBS or StemPro® |
ASC |
Kishimoto et al. 8484 Kishimoto, S; Hattori, H; Nakamura, S; Amano, Y; Kanatani, Y; Tanaka, Y; et al.. Expansion and characterization of human bone marrow-derived mesenchymal stem cells cultured on fragmin/protamine microparticle-coated matrix with fibroblast growth factor-2 in low serum medium. Tissue Eng Part C Methods 2009;15:523-527.
|
8% 4% 2%, 1%, 0.5% HS with FGF2 5 ng/mL (F/P MP-Coated plates) |
not coated HS |
BMSC |
Pytlik et al. 8888 Pytlik, R; Stehlik, D; Soukup, T; Kalbacova, M; Rypacek, F; Trc, T; et al.. The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering. Biomaterials2009;30:3415-3427.
|
10% HS |
10% FBS |
BMSC |
Schallmoser et al. 2020 Schallmoser, K; Strunk, D. Preparation of pooled human platelet lysate (pHPL) as an efficient supplement for animal serum-free human stem cell cultures. J Vis Exp 2009;30:1-4.
|
10% HPL |
- |
MSC |
Bieback et al. 2424 Bieback, K; Ha, VA; Hecker, A; Grassl, M; Kinzebach, S; Solz, H; et al.. Altered gene expression in human adipose stem cells cultured with fetal bovine serum compared to human supplements. Tissue Eng Part A 2010;16:3467-3484.
|
10% HS and 10% aPRP |
10% FBS |
ASC |
Centeno et al. 101101 Centeno, CJ; Schultz, JR; Cheever, M; Freeman, M; Faulkner, S; Robinson, B; et al.. Safety and complications reporting update on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique. Curr Stem Cell Res Ther 2011;6:368-378. * |
10-20% HPL |
- |
BMSC |
Chevallier et al. 5959 Chevallier, N; Anagnostou, F; Zilber, S; Bodivit, G; Maurin, S; Barrault, A; et al.. Osteoblastic differentiation of human mesenchymal stem cells with platelet lysate. Biomaterials2010;31:270-278.
|
5% HPL |
10% FBS |
BMSC |
Felka et al. 5656 Felka, T; Schafer, R; De Zwart, P; Aicher, WK. Animal serum-free expansion and differentiation of human mesenchymal stromal cells. Cytotherapy2010;12:143-153.
|
FFP with platelet concentrate 0.5, 1%, 5%, 10%, 20% and 30% and human plasma-enriched with platelets media 5% and 10% |
10% FBS |
BMSC |
Hartmann et al. 5151 Hartmann, I; Hollweck, T; Haffner, S; Krebs, M; Meiser, B; Reichart, B; et al.. Umbilical cord tissue-derived mesenchymal stem cells grow best under GMP-compliant culture conditions and maintain their phenotypic and functional properties. J Immunol Methods 2010;363:80-89.
|
2% HS |
FBS |
UCMSC |
Horn et al. 6969 Horn, P; Bokermann, G; Cholewa, D; Bork, S; Walenda, T; Koch, C; et al.. Impact of individual platelet lysates on isolation and growth of human mesenchymal stromal cells. Cytotherapy2010;12:888-898.
|
10% HPL |
10% FBS |
MSC |
Ichiyanagi et al. 9494 Ichiyanagi, T; Anabuki, K; Nishijima, Y; Ono, H. Isolation of mesenchymal stem cells from bone marrow wastes of spinal fusion procedure (TLIF) for low back pain patients and preparation of bone dusts for transplantable autologous bone graft with a serum glue. Biosci Trends 2010;4:110-118.
|
Platelet poor plasma 5%, 10% and 20% |
10% FBS |
BMSC |
Lindroos et al. 8787 Lindroos, B; Aho, KL; Kuokkanen, H; Raty, S; Huhtala, H; Lemponen, R; et al.. Differential gene expression in adipose stem cells cultured in allogeneic human serum versus fetal bovine serum. Tissue Eng Part A 2010;16:2281-2294.
|
10%, 15% and 20% HS |
10% FBS |
ASC |
Lucchini et al. 100100 Lucchini, G; Introna, M; Dander, E; Rovelli, A; Balduzzi, A; Bonanomi, S; et al.. Platelet-lysate-expanded mesenchymal stromal cells as a salvage therapy for severe resistant graft-versus-host disease in a pediatric population. Biol Blood Marrow Transplant 2010;16:1293-1301. * |
5% HPL |
- |
MSC |
Salvade et al. 7777 Salvade, A; Della, Mina, P; Gaddi, D; Gatto, F; Villa, A; Bigoni, M; et al.. Characterization of platelet lysate cultured mesenchymal stromal cells and their potential use in tissue-engineered osteogenic devices for the treatment of bone defects. Tissue Eng Part C Methods 2010;16:201-214.
|
5% HPL |
10% FBS |
BMSC |
Abdelrazik et al. 7373 Abdelrazik, H; Spaggiari, GM; Chiossone, L; Moretta, L. Mesenchymal stem cells expanded in human platelet lysate display a decreased inhibitory capacity on T- and NK-cell proliferation and function. Eur J Immunol 2011;41:3281-3290.
|
10% HPL |
10% FBS |
MSC |
Aldahmash et al. 8585 Aldahmash, A; Haack-Sorensen, M; Al-Nbaheen, M; Harknes, L; Abdallah, BM; Kassem, M. Human serum is as efficient as fetal bovine serum in supporting proliferation and differentiation of human multipotent stromal (mesenchymal) stem cells in vitro and in vivo. Stem Cell Rev 2011;7:860-868.
|
2.5%, 5.0% and 10.0% HS |
10% FBS |
BMSC |
Capelli et al. 108108 Capelli, C; Gotti, E; Morigi, M; Rota, C; Weng, L; Dazzi, F; et al.. Minimally manipulated whole human umbilical cord is a rich source of clinical-grade human mesenchymal stromal cells expanded in human platelet lysate. Cytotherapy2011;13:786-801.
|
5% HPL |
- |
UCSC |
Cholewa et al. 4141 Cholewa, D; Stiehl, T; Schellenberg, A; Bokermann, G; Joussen, S; Koch, C; et al.. Expansion of adipose mesenchymal stromal cells is affected by human platelet lysate and plating density. Cell Transplant 2011;20:1409-1422.
|
1%, 2.5%, 5%, 10%, and 20% HPL |
FBS 1%, 2.5%, 5%, 10%, and 20% |
ASC |
Crespo-Diaz et al. 109109 Crespo-Diaz, R; Behfar, A; Butler, GW; Padley, DJ; Sarr, MG; Bartunek, J; et al.. Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability. Cell Transplant 2011;20:797-811.
|
5% HPL |
10% FBS |
BMSC and ASC |
Flemming et al. 7171 Flemming, A; Schallmoser, K; Strunk, D; Stolk, M; Volk, HD; Seifert, M. Immunomodulative efficacy of bone marrow-derived mesenchymal stem cells cultured in human platelet lysate. J Clin Immunol 2011;31:1143-1156.
|
10% HPL |
10% FBS |
BMSC |
Goedecke et al. 9292 Goedecke, A; Wobus, M; Krech, M; Munch, N; Richter, K; Holig, K; et al.. Differential effect of platelet-rich plasma and fetal calf serum on bone marrow-derived human mesenchymal stromal cells expanded in vitro. J Tissue Eng Regen Med 2011;5:648-654.
|
5% and 10% PRP |
10% FBS |
BMSC |
Govindasamy et al. 4747 Govindasamy, V; Ronald, VS; Abdullah, AN; Ganesan, Nathan, KR; Aziz, ZA; Abdullah, M; et al.. Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications. Cytotherapy2011;13:1221-1233.
|
10% HPL |
10% FBS |
DPSC |
Hatlapatka et al. 5050 Hatlapatka, T; Moretti, P; Lavrentieva, A; Hass, R; Marquardt, N; Jacobs, R; et al.. Optimization of culture conditions for the expansion of umbilical cord-derived mesenchymal stem or stromal cell-like cells using xeno-free culture conditions. Tissue Eng Part C Methods 2011;17:485-493.
|
HS 2%, 5%, 10% and HS 2% with 0.5 ng/mL FGF |
FBS |
UCMSC |
Jenhani et al. 6565 Jenhani, F; Durand, V; Ben Azouna, N; Thallet, S; Ben Othmen, T; Bejaoui, M; et al.. Human cytokine expression profile in various conditioned media for in vitro expansion bone marrow and umbilical cord blood immunophenotyped mesenchymal stem cells. Transplant Proc 2011;43:639-643.
|
5%, 10% HPL or 5% HPL and 10% FBS or 10%HPL and 10%FBS |
10% FBS |
BMSC and UCMSC |
Shih et al. 4343 Shih, DT; Chen, JC; Chen, WY; Kuo, YP; Su, CY; Burnouf, T. Expansion of adipose tissue mesenchymal stromal progenitors in serum-free medium supplemented with virally inactivated allogeneic human platelet lysate. Transfusion2011;51:770-778.
|
10% HPL |
10% FBS |
ASC |
Venugopal et al. 106106 Venugopal, P; Balasubramanian, S; Majumda,r AS; Ta, M. Isolation, characterization, and gene expression analysis of Wharton’s jelly-derived mesenchymal stem cells under xeno-free culture conditions. Stem Cells Cloning 2011;4:39-50.
|
5% HS and 2 ng/mL bFGF |
10% FBS |
WJSC |
Xia et al. 7676 Xia, W; Li, H; Wang, Z; Xu, R; Fu, Y; Zhang, X; et al.. Human platelet lysate supports ex vivo expansion and enhances osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. Cell Biol Int 2011;35:639-643.
|
7.5% HPL |
10% FBS |
BMSC |
Jenhani et al. 6565 Jenhani, F; Durand, V; Ben Azouna, N; Thallet, S; Ben Othmen, T; Bejaoui, M; et al.. Human cytokine expression profile in various conditioned media for in vitro expansion bone marrow and umbilical cord blood immunophenotyped mesenchymal stem cells. Transplant Proc 2011;43:639-643.
|
5%,10% HPL 10% FBS 5% HPL |
10% FBS 1 ng/mL fibroblast growth factor 2 (FGF2) |
BMSC |
Behnia et al. 9696 Behnia, H; Khojasteh, A; Soleimani, M; Tehranchi, A; Atashi, A. Repair of alveolar cleft defect with mesenchymal stem cells and platelet derived growth factors: a preliminary report. J Craniomaxillofac Surg 2012;40:2-7. * |
20% HS |
- |
BMSC |
Fekete et al. 1212 Fekete, N; Gadelorge, M; Furst, D; Maurer, C; Dausend, J; Fleury-Cappellesso, S; et al.. Platelet lysate from whole blood-derived pooled platelet concentrates and apheresis-derived platelet concentrates for the isolation and expansion of human bone marrow mesenchymal stromal cells: production process, content and identification of active components. Cytotherapy2012;14:540-554.
|
2.5%, 5%, 10%, 15% and 20% HPL |
FBS 20% |
BMSC |
Fekete et al. 1313 Fekete, N; Rojewski, MT; Furst, D; Kreja, L; Ignatius, A; Dausend, J; et al.. GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC. PLoS One 2012;7:e43255.
|
10% HPL |
- |
BMSC |
Gottipamula et al. 5454 Gottipamula, S; Sharma, A; Krishnamurthy, S; Majumdar, AS; Seetharam, RN. Human platelet lysate is an alternative to fetal bovine serum for large-scale expansion of bone marrow-derived mesenchymal stromal cells. Biotechnol Lett 2012;34:1367-1374.
|
10% HPL (5 to 20% preliminary screening) |
10% FBS |
BMSC |
Kishimoto et al. 9393 Kishimoto, S; Ishihara, M; Mori, Y; Takikawa, M; Hattori, H; Nakamura, S; et al.. Effective expansion of human adipose-derived stromal cells and bone marrow-derived mesenchymal stem cells cultured on a fragmin/protamine nanoparticles-coated substratum with human platelet-rich plasma. J Tissue Eng Regen Med 2013;7:955-964.
|
4%, 2%, 1% and 0.5% PRP or 4%, 2%, 1% and 0.5% HS |
- |
BMSC and ASC |
Kruger et al. 9090 Kruger, JP; Hondke, S; Endres, M; Pruss, A; Siclari, A; Kaps, C. Human platelet-rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells. J Orthop Res 2012;30:845-852.
|
10% HS and 5% PRP |
- |
CSSC |
Lohmann et al. 6161 Lohmann, M; Walenda, G; Hemeda, H; Joussen, S; Drescher, W; Jockenhoevel, S; et al.. Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells. PLoS One 2012;7:e37839.
|
10% HPL |
10% FBS |
BMSC |
Murphy et al. 2222 Murphy, MB; Blashki, D; Buchanan, RM; Yazdi, IK; Ferrari, M; Simmons, PJ; et al.. Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation, chemotaxis, and cryo-preservation. Biomaterials2012;33:5308-5316.
|
1% or 10% PRP |
10% FBS |
UCMSC |
Pisciotta et al. 4848 Pisciotta, A; Riccio, M; Carnevale, G; Beretti, F; Gibellini, L; Maraldi, T; et al.. Human serum promotes osteogenic differentiation of human dental pulp stem cells in vitro and in vivo. PLoS One 2012;7:e50542.
|
10% HS |
10% FBS |
DPSC |
Poloni et al. 113113 Poloni, A; Maurizi, G; Serrani, F; Mancini, S; Discepoli, G; Tranquilli, AL; et al.. Human AB serum for generation of mesenchymal stem cells from human chorionic villi: comparison with other source and other media including platelet lysate. Cell Prolif 2012;45:66-75.
|
5% HPL and HS 10% |
FBS 20% |
CVMS and BMSC |
Stehlik et al. 1111 Stehlik, D; Pytlik, R; Rychtrmocova, H; Kideryova, L; Vesela, R; Kopecny, Z; et al.. Xenogeneic protein-free cultivation of mesenchymal stromal cells - towards clinical applications. Folia Biol (Praha) 2012;58:106-114.
|
10% HS |
- |
BMSC |
Bernardi et al. 6363 Bernardi, M; Albiero, E; Alghisi, A; Chieregato, K; Lievore, C; Madeo, D; et al.. Production of human platelet lysate by use of ultrasound for ex vivo expansion of human bone marrow-derived mesenchymal stromal cells. Cytotherapy2013;15:920-929.
|
10% HPL |
10% FBS |
BMSC |
Griffiths et al. 6868 Griffiths, S; Baraniak, PR; Copland, IB; Nerem, RM; McDevitt, TC. Human platelet lysate stimulates high-passage and senescent human multipotent mesenchymal stromal cell growth and rejuvenation in vitro. Cytotherapy2013;15:1469-1483.
|
2%, 5% and 10% HPL |
FBS 16% |
BMSC |
Hemeda et al. 117117 Hemeda, H; Kalz, J; Walenda, G; Lohmann, M; Wagner, W. Heparin concentration is critical for cell culture with human platelet lysate. Cytotherapy2013;15:1174-1181.
|
10 % HPL |
- |
BMSC and ASC |
Trojahn Kolle et al. 107107 Trojahn Kolle, SF; Oliveri, RS; Glovinski, PV; Kirchhoff, M; Mathiasen, AB; Elberg, JJ; et al.. Pooled human platelet lysate versus fetal bovine serum-investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use. Cytotherapy2013;15:1086-1097.
|
10% HPL |
10% FBS |
ASC |
Kyllonen et al. 4545 Kyllonen, L; Haimi, S; Mannerstrom, B; Huhtala, H; Rajala, KM; Skottman, H; et al.. Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro. Stem Cell Res Ther 2013;4:17.
|
10% HS |
10% FBS |
ASC |
Mojica-Henshaw et al. 118118 Mojica-Henshaw, MP; Jacobson, P; Morris, J; Kelley, L; Pierce, J; Boyer, M; et al.. Serum-converted platelet lysate can substitute for fetal bovine serum in human mesenchymal stromal cell cultures. Cytotherapy2013;15:1458-1468.
|
10% HPL |
10% FBS |
MSC |
Pawitan et al. 8383 Pawitan, J; Wulandari, D; Suryani, D; Damayanti, L; Purwoko, R; Liem, I. Flow cytometry analysis of adipose tissue-derived stem cells that were cultured in various media. Int J Pharm Tech Res 2013;5:1301-1306.
|
5% and 10% PRP 5% and 10% HS |
- |
ASC |
Rojewski et al. 120120 Rojewski, MT; Fekete, N; Baila, S; Nguyen, K; Furst, D; Antwil, D; et al.. GMP-compliant isolation and expansion of bone marrow-derived MSCs in the closed, automated device quantum cell expansion system. Cell Transplant 2013;22:1981-2000.
|
10% HPL |
10% FBS |
BMSC |
Sandor et al. 9898 Sandor, GK; Tuovinen, VJ; Wolff, J; Patrikoski, M; Jokinen, J; Nieminen, E; et al.. Adipose stem cell tissue-engineered construct used to treat large anterior mandibular defect: a case report and review of the clinical application of good manufacturing practice-level adipose stem cells for bone regeneration. J Oral Maxillofac Surg 2013;71:938-950. * |
15% HS |
- |
ASC |
Schallmoser et al. 5858 Schallmoser, K; Strunk, D. Generation of a pool of human platelet lysate and efficient use in cell culture. Methods Mol Biol 2013;946:349-362.
|
10% HPL |
10% FBS |
MSC |
Shanskii et al. 7474 Shanskii, YD; Sergeeva, NS; Sviridova, IK; Kirakozov, MS; Kirsanova, VA; Akhmedova, SA; et al.. Human platelet lysate as a promising growth-stimulating additive for culturing of stem cells and other cell types. Bull Exp Biol Med 2013;156:146-151.
|
10% HPL FBS/HPL ratios: 10/0, 7.5/2.5, 5.0/5.0, 2.5/7.5, and 0/10) |
FBS |
MSC |
Castiglia et al. 2323 Castiglia, S; Mareschi, K; Labanca, L; Lucania, G; Leone, M; Sanavio, F; et al.. Inactivated human platelet lysate with psoralen: a new perspective for mesenchymal stromal cell production in Good Manufacturing Practice conditions. Cytotherapy2014;16:750-763.
|
10% HPL |
10% FBS |
BMSC |
Fekete et al. 1414 Fekete, N; Rojewski, MT; Lotfi, R; Schrezenmeier, H. Essential components for ex vivo proliferation of mesenchymal stromal cells. Tissue Eng Part C Methods 2014;20:129-139.
|
5%, 10% and 20% HPL 5%, 10% and 20% HS |
FBS 5%, 10% and 20% |
BMSC |
Gottipamula et al. 104104 Gottipamula, S; Ashwin, KM; Muttigi, MS; Kannan, S; Kolkundka, U; Seetharam, RN. Isolation, expansion and characterization of bone marrow-derived mesenchymal stromal cells in serum-free conditions. Cell Tissue Res 2014;356:123-135.
|
2.5 % HS |
10% FBS |
BMSC |
Iudicone et al. 6666 Iudicone, P; Fioravanti, D; Bonanno, G; Miceli, M; Lavorino, C; Totta, P; et al.. Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells. J Transl Med 2014;12:28.
|
10% HPL |
10% FBS |
BMSC |
Koellensperger et al. 4242 Koellensperger, E; Bollinger, N; Dexheimer, V; Gramley, F; Germann, G; Leimer, U. Choosing the right type of serum for different applications of human adipose tissue-derived stem cells: influence on proliferation and differentiation abilities. Cytotherapy2014;16:789-799.
|
HS 2%, 10%; PPP 2%, 10%; PL 2%, 10% |
10% FBS |
ASC |
Martins et al. 5353 Martins, TM; de Paula, AC; Gomes, DA; Goes, AM. Alkaline phosphatase expression/activity and multilineage differentiation potential are the differences between fibroblasts and orbital fat-derived stem cells--a study in animal serum-free culture conditions. Stem Cell Rev 2014;10:697-711.
|
10% HS |
10% FBS |
OFSC |
Muraglia et al. 1919 Muraglia, A; Ottonello, C; Spano, R; Dozin, B; Strada, P; Grandizio, M; et al.. Biological activity of a standardized freeze-dried platelet derivative to be used as cell culture medium supplement. Platelets2014;25:211-220.
|
5% HPL |
10% FBS |
BMSC |
Pham et al. 2525 Pham, PV; Vu, NB; Pham, VM; Truong, NH; Pham, TL; Dang, LT; et al.. Good manufacturing practice-compliant isolation and culture of human umbilical cord blood-derived mesenchymal stem cells. J Transl Med 2014;12:56.
|
2%, 5%, 7% and 10% aPRP |
10% FBS |
UCMSC |
Yamauchi et al. 7979 Yamauchi, T; Saito, H; Ito, M; Shichinohe, H; Houkin, K; Kuroda, S. Platelet lysate and granulocyte-colony stimulating factor serve safe and accelerated expansion of human bone marrow stromal cells for stroke therapy. Transl Stroke Res 2014;5:701-710.
|
5% HPL |
10% FBS |
BMSC |
Antoninus et al. 1717 Antoninus, AA; Widowati, W; Wijaya, L; Agustina, D; Puradisastra, S; Sumitro, SB; et al.. Human platelet lysate enhances the proliferation of Wharton’s jelly-derived mesenchymal stem cells. Biomarkers and Genomic Med 2015;7:87-97.
|
HPL 0.031 mg/mL, 0.063 mg/mL, 0.125 mg/mL, and 0.250 mg/mL |
20% FBS |
WJSC |
Budiyanti et al. 4949 Budiyanti, E; Liem, I; Pawitan, J; Wulandari, D; Jamaan, T; Sumapradja, K. Umbilical cord derived mesenchymal stem cell proliferation in various platelet rich plasma and xeno-material containing medium. Int J Res Pharm Sci 2015;6:7-13.
|
PRP 10% |
MesenCult®
|
UCSC |
Castren et al. 1818 Castren, E; Sillat, T; Oja, S; Noro, A; Laitinen, A; Konttinen, YT; et al.. Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum. Stem Cell Res Ther 2015;6:167.
|
AB-plasma 2.5% + HPL 0.5% |
10% FBS |
BMSC |
Jonsdottir-Buch et al. 1515. Jonsdottir-Buch, SM; Sigurgrimsdottir, H; Lieder, R; Sigurjonsson, OE. Expired and pathogen-inactivated platelet concentrates support differentiation and immunomodulation of mesenchymal stromal cells in culture. Cell Transplant 2015;24:1545-1554.
|
10% HPL |
- |
BMSC |
Laitinen et al. 5555 Laitinen, A; Oja, S; Kilpinen, L; Kaartinen, T; Moller, J; Laitinen, S; et al.. A robust and reproducible animal serum-free culture method for clinical-grade bone marrow-derived mesenchymal stromal cells. Cytotechnology2015;68:891-906.
|
5% and 10% HPL |
10% FBS |
BMSC |
Laner-Plamberger et al. 6767 Laner-Plamberger, S; Lener, T; Schmid, D; Streif, DA; Salzer, T; Oller, M; et al.. Mechanical fibrinogen-depletion supports heparin-free mesenchymal stem cell propagation in human platelet lysate. J Transl Med 2015;13:354.
|
10% HPL |
10% HPL |
BMSC and UCMSC |
Li et al. 5757 Li, CY; Wu, XY; Tong, JB; Yang, XX; Zhao, JL; Zheng, QF; et al.. Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy. Stem Cell Res Ther 2015;6:55.
|
5% HPL |
- |
BMSC and ASC |
Luzzani et al. 5252 Luzzani, C; Neiman, G; Garate, X; Questa, M; Solari, C; Fernandez, Espinosa, D; et al.. A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement. Stem Cell Res Ther 2015;6:6.
|
10% HPL |
10% FBS |
UCMSC and iPS |
Oikonomopoulos et al. 4646 Oikonomopoulos, A; van Deen, WK; Manansala, AR; Lacey, PN; Tomakili, TA; Ziman, A; et al.. Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media. Sci Rep 2015;5:16570.
|
10% HPL |
10% FBS |
BMSC and ASC |
Paula et al. 4444 Paula, AC; Martins, TM; Zonari, A; Frade, SP; Angelo, PC; Gomes, DA; et al.. Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation. Stem Cell Res Ther 2015;6:76.
|
HS 10% |
10% FBS |
ASC |
Pawitan et al. 9191 Pawitan, J; Feroniasanti, L; Kispa, T; Dilogo, I; Fasha, I; Kurniawati, T; et al.. Simple method to isolate mesenchymal stem cells from bone marrow using xeno-free material: A preliminary study. Int J Pharm Tech Re 2015;7:354-359.
|
PRP 10% |
10% FBS |
BMSC |
Riordan et al. 105105 Riordan, NH; Madrigal, M; Reneau, J; de Cupeiro, K; Jimenez, N; Ruiz, S; et al.. Scalable efficient expansion of mesenchymal stem cells in xeno free media using commercially available reagents. J Transl Med 2015;13:232.
|
5%, 7.5% and 10% HPL |
10% FBS |
WJSC |