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MARTINS, João Ricardo; CORREA, Bartolomeu Lima and CERESER, Victor Hermes. A comparative study between ELISA and indirect imunofluorescent (IFI) tests to detect antibodies against Babesia bovis. Cienc. Rural [online]. 1996, vol.26, n.1, pp. 115-118. ISSN 0103-8478. http://dx.doi.org/10.1590/S0103-84781996000100021.
Performance of an ELISA kit for detecting antibodies to Babesia bovis, a bovine haemoparasite, was compared with the Indirect Fluorescent Antibody Test (IFAT) used in serological routine. Over 97 sera samples from a free tick area, ELISA showed an specificity of 98.9% against 97.9% obtained by IFA T. Both tests showed a sensitivity of 100% when compared over 22 sera samples from experimentally infected bovine with B. bovis. Results obtained with 1560 field samples showed an agreement of 90.1% (1406/1560) between positive and negativo samples while 4.2% (66) were positives to IFAT and negatives to ELISA, and 5.6% (88) owere negatives to IFAT and positives to ELISA.
Keywords : serology; Babesia bovis; ELISA; IFAT.