Abstract

BENNEMANN, Paulo Eduardo; BORTOLOZZO, Fernando Pandolfo; WENTZ, Ivo  and  CARDOSO, Marisa Ribeiro de Itapema. Sperm motility and acrossomal integrity in liquid boar semen dosis inoculated with Esecherichia coli and Staphylococcus aureus. Cienc. Rural [online]. 2000, vol.30, n.2, pp. 313-318. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782000000200019.

Twelve ejaculates were collected in the most aseptic manner and distributed in seven treatments (control group T1). Semen were inoculated with S. aureus (T2, T3 e T4) or E. coli (T5, T6 e T7) in three concentrations (5 x 10⁵, 5 x 10⁶ and 5 x 10⁷ CFU/ml). The sperm motility, the percentage of normal apical range (NAR), the pH and the number of colony unit former (CFU/ml) of bacteria, for 96 hours, were evaluated. The bacterial development was decreasing during the first 96 hours. Except for the treatment with the inoculation of 5 x 10⁷ CFU of E. coli/ml, there was no significant effect of the bacteria on the sperm motility (p>0.05). Also, there was no significant effect (p>0.05) of the S. aureus or E. coli on the percent of NAR and the pH during the 96 hours. When compared to the control group, only the inoculation of 5 x 10⁷ CFU/ml of E. coli differed at the 96 hours in relation to the percentage of NAR (p<0.05). There was no correlation among the sperm motility, NAR, pH and CFU.

Keywords : semen; acrosome; Escherichia coli; Staphylococcus aureus.