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RUSSOWSKI, Denise and NICOLOSO, Fernando Teixeira. Nitrogen and phosphorus on growth of brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] in vitro cultured plants. Cienc. Rural [online]. 2003, vol.33, n.1, pp.57-63. ISSN 0103-8478. http://dx.doi.org/10.1590/S0103-84782003000100009.
Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] has expressionable medicinal properties, and therefore, nowadays it is extensively exploited either by the herb collectors as well as the pharmaceutical industry. The aim of this work was to characterize the effect of N and P concentrations from the MS medium on growth of P. glomerata in vitro cultured plants. 1-node segment without leaves, from in vitro cultivated plants, were grown on five levels (0, 25, 50, 100, and 150% of the strength of the MS medium) of N and P. After 15 days of inoculation, root number and rooting percentage are greater on N and P levels of the MS medium with 50% strength. After 40 days of inoculation, growth, based on sprout height, number of nodal segments per plant, leave area index per plant, number of leaves per plant, dry weight of aerial parts, roots and of the whole plant, is greater when the N and P levels of the MS medium is near to 80% strength.
Keywords : micropropagation; macronutrient; tissue culture; Amaranthaceae.