On-line version ISSN 1678-4596
MALDANER, Joseila et al. Sucrose and nitrogen on in vitro multiplication of Pffafia glomerata (Spreng.) Pedersen. Cienc. Rural [online]. 2006, vol.36, n.4, pp. 1201-1206. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782006000400024.
Pfaffia glomerata (Spreng.) Pedersen, known as brazilian ginseng, is an extensively used plant in folk medicine due to its phytotherapic characteristics. This work aimed to determine the best combination of sucrose (30, 45 and 60 g L-1) and nitrogen (50, 75, 100 and 125% of the strength of MS medium) on micropropagation of P. glomerata. In 30 days of cultivation, sucrose ranging from 40 to 45g L-1 and nitrogen at 50% increased the height and the number of nodes. The number of shoots was greater at concentration of 55g L-1 sucrose combined with 70% N. The dry matter of roots, aerial parts, and of the whole seedling was increased on 50% N and 45g L-1 sucrose. Altogether the halved concentration of nitrogen and the increased concentration of sucrose to 45g L-1 increase the height, the number of nodes and the shoots as well as biomass production improved due to an enhancemednt of carbon use.
Keywords : Ginseng brasileiro; Amaranthaceae; in vitro culture; carbohydrate; mineral nutrient.