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Ciência Rural

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Abstract

PEIXOTO, Ana Rosa; MARIANO, Rosa de Lima Ramos  and  VIANA, Ivanise Oliveira. Semi-selective medium for isolation of Xanthomonas campestris pv. viticola. Cienc. Rural [online]. 2006, vol.36, n.4, pp. 1317-1320. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782006000400045.

Bacterial canker caused by Xanthomonas campestris pv. viticola is the most important bacterial disease of grapevine in Submédio São Francisco. The isolation of X. campestris pv. viticola of infected plant tissues is impaired by the presence of bacterial contaminants including Microbacterium barkeri. Aiming to formulate a semi-selective medium 22 X. campestris pv. viticola isolates were tested in relation to 30 antibiotics. The semi-selective NYDAM medium (meat extract 3, peptone 5, glucose 10, yeast extract 5, agar 18 and ampicilin 0.1 in gL-1) inhibited M. barkeri and plant pathogenic bacteria allowing X. campestris pv. viticola isolation from hosts naturally infected in the field.

Keywords : bacterial canker of grapevine; Vitis sp.; antibiotics.

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