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Ciência Rural

On-line version ISSN 1678-4596


ARAUCO, Luis Ricardo Romero; DE STEFANI, Marta Verardino; NAKAGHI, Laura Satiko Okada  and  OLIVEIRA-BAHIA, Verônica Regina Lobato de. Histology of kidney, liver and intestine of bullfrog tadpoles (Rana catesbeiana) fed with diets containing propolis. Cienc. Rural [online]. 2007, vol.37, n.5, pp.1436-1441. ISSN 1678-4596.

This research was aimed at verifying the occurrence of possible alterations in liver, kidney and intestine of bullfrog tadpoles in stage 42 fed with diets containing different concentrations (0.0; 0.2; 0.5; 1.0 and 1.5%) of propolis hydroalcoholic extract. The experiment was carried out in laboratory of Aquatic Organisms Nutrition from Aquaculture Center of UNESP, where 1,400 tadpoles in stage 26 were used and distributed in twenty experimental aquariums. In the end of experiment (60 days) three tadpoles from each repetition were sacrificed and kidney, liver and intestine samples were collected to processing of histological slices in Histology´s Laboratory pertaining to Department of Morphology and Physiology from FCAV - UNESP. Samples were fixed, dehydrated, stained in HE, analysed, photomicrographed and thickness of intestinal epithelium was measured. Histological disturbances in tadpoles´s intestine, kidneys and liver were not observed. Thickness of intestinal epithelium from the same ones was not influenced (P>0.05) by propolis concentrations.

Keywords : frogculture; histology; propolis; organs.

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