SciELO - Scientific Electronic Library Online

 
vol.38 issue7Ascitic condemnations in broilers slaughtered under Federal Inspection between 2002 and 2006 in Rio Grande do Sul, BrazilIntrauterine artificial insemination of gilts using cryopreserved boar semen with dimetilacetamide and glycerol author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Ciência Rural

Print version ISSN 0103-8478

Abstract

TERRACIANO, Paula Barros et al. Cryopreservation of equine spermatozoa comparing different freezing rates combined with commercial extenders: laboratorial analysis. Cienc. Rural [online]. 2008, vol.38, n.7, pp. 1972-1977. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782008000700027.

During semen cryopreservation, sperm cells were submitted to several deleterious events leading to membrane damage which result in fertility decrease. This study was designed to compare the effects of two freezing techniques (conventional and automated), and the use of two commercial extenders as cryoprotectants (FR-5® and Botu-Crio®) on total and progressive motility, integrity and functionality of spermatic membranes during the cryopreservation of equine semen. Twenty ejaculates from two stallions were analyzed. The total and progressive motility of fresh and post-thawing semen samples were evaluated by patterns assays. Function of plasmatic membrane was measured by the hipoosmotic swelling test. Integrity of plasmatic membrane was evaluated using carboxifluorescein diacatate and iodidium propide fluorescent probes. There were significant differences between the two freezing techniques and/or between cryoprotectants for all assessed parameters. The combination of Botu-Crio® and automated curves showed better results on total and progressive post-thawing motility. The extender Botu-Crio®, alone, showed to better preserve the membrane integrity and function.

Keywords : semen; equine; cryopreservation; spermatozoon cell; cryoprotectants.

        · abstract in Portuguese     · text in Portuguese     · pdf in Portuguese