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On-line version ISSN 1678-4596
OLIVEIRA, Tatiana Catelan de; SCAVONE, Alessandra Regina Freixo; MACHADO, Márcia Rita Fernandes and MAZZUCATTO, Bárbara Cristina. Experimental cystoplasty in rabbits (Oryctolagus cuniculus) with bovine peritoneum conserved in 98% glycerol. Cienc. Rural [online]. 2008, vol.38, n.8, pp.2218-2224. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782008000800020.
Bovine peritoneum was utilized to replace a section of ventral face of the bladder of New Zealand rabbits, adult and entire males (n=12) with the purpose of evaluating the tissue repair process in regard of its biocompatility, scar formation ability and possible complications. The animals were euthanized at 7, 14, 30 and 60 days post operative, three in each period, by the use of sodium thiopental (200mg kg-1), followed by macroscopic evaluation and histopathological analysis of the implant interface with the native tissue. Macroscopically it was observed, in all time periods, structure adherence to the implant site, presence of calculi and absence of rejection signs. Under light microscopy at the 7th, 14th and 30th observation days, the implant was present; a mixed intense inflammatory reaction could be observed, neovascularization, fiberplasic process and rare muscle fibers, however the epithelium and sheath werent also reconstituted. At the 60th day of evaluation the implant wasnt anymore present and all vesicle layers were repaired. The implant was biocompatible and provided the mainframe for orientation and development of the tissue layers of the bladder, through repairing processes thus reestablishing the organs structure.
Keywords : implant; biological membrane; bladder; rabbits.