SciELO - Scientific Electronic Library Online

 
vol.38 issue8Ecological aspects of small strongylids in the Paraíba Valley Region, State of São Paulo, BrazilMetanalysis for carcass traits of cattle from different genetic groups author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Ciência Rural

Print version ISSN 0103-8478

Abstract

SILVA, Karen Mascaro Gonçalves da et al. Effect of pyruvate and trolox added to the extender used for freezing fertile and subfertile stallion semen. Cienc. Rural [online]. 2008, vol.38, n.8, pp. 2271-2277. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782008000800028.

The objective of this research was to evaluate the effect of pyruvate and trolox on the thawed sperm quality. For freezing, antioxidants were added to INRA 82-HEPES extender to protect sperm from the deleterious effects of oxidative stress, according to the treatments: T1= INRA82-HEPES without antioxidants; T2= INRA82-HEPES + 2mM of pyruvate and T3= INRA82-HEPES + 120 mM de trolox. The thawed semen samples were evaluated according to the total (MT) and progressive (MP) motility, integrity of plasma and acrossomal membrane, DNA integrity, membrane stability and mitochondrial membrane potential (Δψm). It was observed that the addition of pyruvate resulted in a significantly higher total sperm motility (P<0.05) to those obtained with trolox (9.17 and 14.5%, respectively). It can be concluded that the addition of pyruvate improves sperm motility (18.92 and 19.0%, respectively) in samples from fertile and sub-fertile stallions submitted to freezing.

Keywords : trolox; pyruvate; freezing; spermatic viability; equine.

        · abstract in Portuguese     · text in Portuguese     · pdf in Portuguese