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On-line version ISSN 1678-4596
FREITAS, Theonys Diógenes et al. Production and characterization of monoclonal antibodies against Clostridium perfringens Type D epsilon toxin. Cienc. Rural [online]. 2009, vol.39, n.1, pp. 269-271. Epub Sep 04, 2008. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782008005000051.
Clostridium perfringens type D is the aetiological agent of enterotoxemia in ruminants. The disease is caused by epsilon toxin characterized by cardiac, pulmonary, kidney and brain edema. Monoclonal antibodies were produced by using myeloma cell line P3-X63-Ag8 653 fused with spleen cells from Balb/c mice, immunized with epsilon toxoid of C. perfringens type D. Six hybrids were established secreting monoclonal antibodies of the IgM class and IgG3 subclass.
Keywords : Clostridium perfringens; epsilon toxin; monoclonal antibodies.