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Ciência Rural

Print version ISSN 0103-8478On-line version ISSN 1678-4596


SILVA, Flávio de Oliveira et al. Proteases profile of skin wounds treated with lectin from Canavalia brasiliensis seeds. Cienc. Rural [online]. 2009, vol.39, n.6, pp.1808-1814. ISSN 0103-8478.

The objective of the present study was determining the proteases profile of cutaneous healings treated with free and conjugated lectin of Canavalia brasiliensis (ConBr) and their specific sugar. An aseptic wound was produced in the thoracic area of the mice (n=120), divided according to the employed treatment: NaCl Group (150mM NaCl), manose Group (100mM manose), ConBr Group (100µg mL-1 ConBr) and ConBr/manose Group (solution containing 100µg mL-1 ConBr prepared in 100mM manose). Samples of the injured area were collected for determination of proteases profile and collagenolytic activity on 2nd, 7th e 12th days after the surgery. Electrophoresis SDS-PAGE demonstrated proteins with molecular mass of 67kDa in all groups. Group IV presented the highest collagenolytic activity on the 12th day post surgery. ConBr lectin influenced proteases expression with collagenolytic activity thus being able to intervene on skin wound healing in mice.

Keywords : Canavalia brasiliensis; lectins; metalloproteinases; wound healing; wound repair.

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