Abstract

SALVATO, Fernanda  and  CARVALHO, Mayra Costa da Cruz Gallo de. Methods and strategies in proteomics and their applications in plants. Cienc. Rural [online]. 2010, vol.40, n.3, pp. 727-734.  Epub Feb 26, 2010. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782010005000018.

The implementation of mass spectrometry (MS) for peptides (MS) and amino acids (tandem MS or MS/MS) analysis allowed the identification of hundreds of proteins in single experiments. A number of different strategies are current available for sample fractioning and purification, proteins identification, quantification, post-translational modifications (PTM) and interaction analyses. In this way, the proteomics open up new perspectives in plant biology with emphasis on studies of genetic variability, physiological stresses and plant development.

Keywords : proteomics; protein analysis; interactomics; plants.