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On-line version ISSN 1678-4596
CARVALHO, Mayra Costa da Cruz Gallo de and SILVA, Danielle Cristina Gregorio da. Next generation DNA sequencing and its applications in plant genomics. Cienc. Rural [online]. 2010, vol.40, n.3, pp.735-744. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782010000300040.
The next-generation DNA sequencing technologies are a powerful alternative to studies in structural and functional genomics. In plant genomics studies, the work with these new platforms has been used for the sequencing of transcripts, re-sequencing, and the de novo sequencing of plastid genomes. This research details the technological principles of the next-generation DNA sequencing platforms most used and reviews its application in structural and functional plant genomics.
Keywords : short reads; res-sequencing; de novo sequencing; plants structural genomics; plants transcriptomics; functional genomics.