Abstract

PINHO, Daiane Schmidt de et al. In vitro regeneration of melon, cv. 'Gaúcho'. Cienc. Rural [online]. 2010, vol.40, n.5, pp. 1083-1089.  Epub May 28, 2010. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782010005000080.

The aim of the present study was to optimize a cv. Gaucho melon cotyledon explant regeneration protocol, evaluating the germination media composition and the explant exposition period in these media. For this purpose, seeds were germinated in semi-solid MS medium containing either BAP or ANA, and without growth regulators. The seeds were kept in these media for one, two, three or four days; afterwards, their cotyledons were inoculated in MS medium containing different BAP concentrations (0.5; 0.9; 1.5 and 2.0mg L^-1). The highest regeneration rates occurred with cotyledons from seeds which had been kept for one or two days in media containing 0.5 and 0.9mg L^-1 BAP. Both the increase in the explant exposition time in germination media and high BAP concentrations in regeneration media decreased explant organogenic capacity, and increased callus formation.

Keywords : Cucumis melo L.; culture medium; morphogenesis.