Abstract

COSTA, Geisa Moreira da; NEPOMUCENO, Cristina Ferreira  and  SANTANA, José Raniere Ferreira de. In vitro propagation of Erythrina velutina. Cienc. Rural [online]. 2010, vol.40, n.5, pp. 1090-1096.  Epub June 07, 2010. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782010005000084.

Erythrina velutina (mulungu) is a native plant of the semi-arid region that can be propagated via tissue culture, which may contribute to the rapid multiplication of this species, reducing the possibility environmental impacts. The objective of this study was to establish a protocol for micropropagation of mulungu using aseptic explants seedlings grown in vitro. For the induction of shoots, , nodal segments and cotyledons were used as explants hypocontyl and were inoculated in a WPM culture medium supplemented with different concentrations of BAP and NAA. The rooting of shoots was achieved in a WPM medium supplemented with 0.0; 2.46; 4.92; 9.88µM IBA. The best answer to the multiplication was obtained in medium supplemented with 17.76µM BAP using nodal segment or cotyledonary node explants. The shoots rooted in all conditions tested, including the culture medium without auxin.

Keywords : Mulungu; tissue culture; micropropagation; plant growth regulator.