Print version ISSN 0103-8478
ZOCCHE, Fernando; BASTOS, Caroline Peixoto and SILVA, Wladimir Padilha da. Detection of genes of egc cluster in Staphylococcus aureus isolated from foods of animal origin. Cienc. Rural [online]. 2010, vol.40, n.5, pp. 1134-1140. ISSN 0103-8478. http://dx.doi.org/10.1590/S0103-84782010000500021.
The aim of this study was to detect, through PCR usage, the genes which encodes staphylococcal enterotoxins and which belongs to egc cluster (seg, sei, selm, seln and selo) in S. aureus isolated from different foods of animal origin and correlate their presence with the strain origin. Forty-one strains of S. aureus from different sources (chicken meat, raw milk, sausage meat and cheese) were evaluated through PCR by amplifying a fragment of 3375bp (called partial egc), which was used as a marker for the presence of cluster, and fragments of individual genes belonging to egc cluster. There is presence of the egc cluster in strains of S. aureus isolated from foods of animal origin, however, different genotypes could be observed depending on the isolation source. The occurrence of strains isolated from chicken meat that had all the genes of the cluster was high; however, in the strains isolated from the other foods, such occurrence has been reduced.
Keywords : egc cluster; staphylococcal enterotoxins; contamination; foods of animal origin; PCR.