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Ciência Rural

Print version ISSN 0103-8478


RIZZO, Huber et al. Mollicutes isolation and PCR on ovine vaginal mucous and its association with reproductive problems in Piedade, SP, Brazil. Cienc. Rural [online]. 2011, vol.41, n.2, pp.324-329.  Epub Feb 11, 2011. ISSN 0103-8478.

It was evaluated the presence of Mycoplasma spp, Ureaplasma spp and Acholeplasma laidlawiii in 60 samples of ovine vaginal mucous with the presence or absence of vulvovaginitis in the specific exam of the reproductive tract. The microorganisms were characterized based on bacteriological culture and DNA detection by Polymerase Chain Reaction (PCR) with specific primers to Mollicutes (GPO and MGSO), Ureaplasma (UGPF and UGPS) and Acholeplasma laidlawii (UNI and ACH3). The presence of mycoplasmas could not be associated with reproductive disorders in animals. The PCR to Acholeplasma laidlawii detected only one positive sample. However, all isolations of Ureaplasma spp were from animals presenting reproductive disorders, suggesting a possible involvement of this agent in reproductive diseases.

Keywords : PCR; mycoplasmas; ureaplasmas; sheep; vulvovaginitis.

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