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Ciência Rural

Print version ISSN 0103-8478

Abstract

CAMILLO, Giovana et al. Indirect fluorescent antibody test to detection of Neospora caninum antibodies in samples of bulk milk. Cienc. Rural [online]. 2011, vol.41, n.9, pp. 1600-1604. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782011000900018.

The aim of this study was to determine the presence of anti-N. caninum in the bulk milk samples by indirect immunofluorescence assay (IFAT). For this, it was collected samples of milk from refrigerated tanks from 36 herds, of which 14 were selected for sampling of individual blood serum and milk of cows. It was found concordance for 12 of the 14 herds selected for samples of individual animals, compared to the detection of antibodies in the bulk milk samples. Concordance was of 76% when comparing the results of the pooled sample with the results of individual milk. It was observed low antibody titers (50) in the serum of animals positive whose results in IFA of bulk milk and blood were discordant. Thus, with the results of this study, it can be concluded that the use of serological tests for detection of anti-N. caninum in the bulk milk samples from the IFAT could be an alternative diagnosis for the screening of positive herds as well as for epidemiological studies.

Keywords : IFAT; neosporosis; dairy herds; diagnostic.

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