On-line version ISSN 1678-4596
DIAS, Natanael Lamas; FONSECA JUNIOR, Antônio Augusto; RODRIGUES, Daniel Sobreira and CAMARGOS, Marcelo Fernandes. Enzootic bovine leukosis real time PCR. Cienc. Rural [online]. 2012, vol.42, n.8, pp.1434-1439. Epub July 10, 2012. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782012005000053.
The goal of this research was to validate a Plexor® real time Polymerase Chain Reaction (qPCR) for Enzootic Bovine Leukosis (EBL) diagnosis by comparison with methods recommend by the World Animal Health Organization (OIE). The qPCR was compared with two other techniques: the nested PCR (nPCR) and to the agar gel immunodiffusion (AGID). Of 82 qPCR and nPCR analysed samples, 79 presented concordant results, being the concordance classified by Kappa Index as high. Between the PCRs and AGID, the number of concordant results was 71 and 69, out of 82, to qPCR and nPCR, respectively, being the concordance classified as considerable, in both cases. The qPCR presented high specificity and sensitivity values. The observed qPCR negative and positive predictive values show that the qPCR has a high capacity to correctly classify positive and negative results. The qPCR was not able to detect three positive animals and has a lightly higher cost than the nPCR. However, the qPCR its faster, less prone to contamination, has a high sensitivity and does not use or generate carcinogenic residues. We conclude that the qPCR may be used to replace the OIE nPCR technique in routine diagnosis in areas where EBL is endemic, as is the case of Brazil.
Keywords : BLV; validation; qPCR; nPCR; AGID.