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Ciência Rural

Print version ISSN 0103-8478

Abstract

TOSCAN, Gustavo et al. Detection of nucleic acids in tissues of gerbils submitted to acute infection with Neospora caninum. Cienc. Rural [online]. 2012, vol.42, n.10, pp. 1865-1871.  Epub Aug 28, 2012. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782012005000079.

Neospora caninum is a protozoan of great importance for livestock, mainly by causing reproductive diseases in cattle. Gerbils (Meriones unguiculatus) may be a model to reproduce experimental acute neosporosis of cattle. In this study two groups of gerbils were formed (n=10) and gerbils were inoculated with N. caninum tachyzoites (NC-1 strain) at two different doses appropriate to cause acute neosporosis: 5x106 tachyzoites ml-1 (G1) or 5x105 tachyzoites ml-1 (G2). Brain, spinal cord, heart, lung, liver, kidneys and spleen were collected and PCR was performed using samples of these tissues and organs. DNA of N. caninum was detected in at least five tissues for most animals (12/20; 60%) considering both groups. In group 1, the frequency of DNA detection, evaluating all samples, was higher (52/70; 74.28%) compared to group 2 (38/70; 54.28%). These results showed that the protozoan replicated efficiently after inoculation spreading by several tissues. Also, was demonstrated that gerbils can be used as model for acute infection with N. caninum showing clinical signs of neosporosis.

Keywords : N. caninum; acute infections; tissue distribution.

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