versión On-line ISSN 1678-4596
ROSA, Claudia Severo da et al. Purification and characterization of hyaluronic acid from chicken combs. Cienc. Rural [online]. 2012, vol.42, n.9, pp.1682-1687. Epub 19-Jul-2012. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782012005000056.
Hyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. The umbilical cord and the chicken comb are some of the tissues richest in this polysaccharide. The profit from obtaining HA from the combs of slaughtered animals is particularly attractive. This work aimed to extract, purify, and characterize HA. The glycosaminoglycan concentration in the chicken comb was found to be about 15µg of hexuronic acid mg-1 of dry tissue. Fractionation using ion exchange chromatography and subsequent identification of the fractions by agarose gel electrophoresis showed that HA corresponded to 90% of the total amount of extracted glycosaminoglycans.
Palabras clave : glycosaminoglycans; chicken combs; hexuronic acid.