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Ciência Rural

Print version ISSN 0103-8478

Abstract

LAJUS, Cristiano Reschke et al. Leaf tissues proportion and chemical composition of Axonopus jesuiticus x A. scoparius as a function of pig slurry application. Cienc. Rural [online]. 2014, vol.44, n.2, pp.276-282.  Epub Dec 10, 2013. ISSN 0103-8478.  http://dx.doi.org/10.1590/S0103-84782013005000154.

This study aimed to evaluate the chemical and anatomical attributes of leaves of giant missionary grass to application of 0, 62, 124, 186, 248 and 310m3 ha-1 of pig slurry. At 83 days after the last application of fertilizer, the leaf blades were collected, fixed in FAA 70%, sectioned, stained, photographed and digitalized. The transversal section of leaf blades were evaluated for proportion of epidermis, lignified vascular tissue + sclerenchyma, non-lignified vascular tissue and parenchyma with an image-processing system calibrated to 1mm pixel-1. Leaf samples were analyzed for crude protein, acid detergent fiber, neutral detergent fiber and hemicellulose content by near infrared reflectance spectroscopy. The pig slurry application up to 310m3 ha-1 significantly increased the percentage of crude protein, parenchyma, epidermis, non-lignified vascular tissue and hemicellulose, while decreasing the percentage of acid detergent fiber and lignified vascular tissue + sclerenchyma. The Pearson's correlation was positive between crude protein and non-lignified vascular tissue, and between acid detergent fiber and lignified vascular tissue + sclerenchyma. The percentage of hemicellulose was positively correlated with epidermis, parenchyma and non-lignified vascular tissue. A negative correlation between acid detergent fiber and epidermis, parenchyma and non-lignified vascular tissue was observed.

Keywords : crude protein; epidermis; fiber; lignified vascular tissue + sclerenchyma; parenchyma.

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