Acessibilidade / Reportar erro
MICROBIOLOGY • Cienc. Rural 46 (5) • May 2016 • https://doi.org/10.1590/0103-8478cr20150454   copy
This document is related to:

Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain

Prevenção da infecção por Clostridium difficile em hamsters usando uma estirpe não toxigênica

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

The present study aimed to evaluate five non-toxigenic strains ofClostridium difficile (NTCD) in vitro and to select one strain to preventC. difficile (CDI) infection in hamsters (Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain ofC. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain ofC. difficile (Z31) was able to prevent CDI in all animals that received it.

Key words:
diarrhea; Clostridium difficile- associated diseases; colitis

Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br
Acompanhe os números deste periódico no seu leitor de RSS