Micropropagation of <i>Stevia rebaudiana</i> plants

Rokosa, Marta Teresa; Kulpa, Danuta

doi:10.1590/0103-8478cr20181029

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BIOLOGY • Cienc. Rural 50 (1) • 2020 • https://doi.org/10.1590/0103-8478cr20181029 copy

Micropropagation of Stevia rebaudiana plants

Micropropagação de plantas de Stevia rebaudiana

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

The aim of the study was to develop optimum composition of plant growth regulators in media for the propagation and rooting of shoots of stevia (Stevia rebaudiana Bertoni) in in vitro cultures. Single-node shoot fragments obtained from plants propagated on MS medium were placed onto media supplemented with: BAP, 2iP and KIN at concentrations: 0.5, 1, 2 and 5 mg∙dm^-3, whereas at the rooting stage with addition of: IAA, IBA and NAA at concentrations 1, 2, 4 and 8 mg∙dm^-3. The highest number of shoots and leaves was reported for plants propagated on MS medium enriched with 0.5 mg∙dm^-3 BAP. The greatest number of the longest roots was developed by stevia on the MS medium enriched with 1 mg∙dm^-3 IAA.

Key words:
micropropagation; plant growth regulators; cytokinins; auxins; steviosides

Authorship

Marta Teresa Rokosa ^*E-mail: marta.rokosa@zut.edu.pl. *Corresponding author.

Laboratory of Plant Physiology and Entomology, Department of Bioengineering, Faculty of Environmental Management and Agriculture, West Pomeranian University of Technology in Szczecin, ul. Juliusza Słowackiego 1770-953 Szczecin, Poland. West Pomeranian University of Technology in SzczecinPolandSzczecin, PolandLaboratory of Plant Physiology and Entomology, Department of Bioengineering, Faculty of Environmental Management and Agriculture, West Pomeranian University of Technology in Szczecin, ul. Juliusza Słowackiego 1770-953 Szczecin, Poland.

http://orcid.org/0000-0002-8855-2365

Danuta Kulpa

Department of Genetics, Plant Breeding and Biotechnology, Faculty of Environmental Management and Agriculture West Pomeranian University of Technology in Szczecin, Szczecin, Poland.West Pomeranian University of Technology in SzczecinPolandSzczecin, PolandDepartment of Genetics, Plant Breeding and Biotechnology, Faculty of Environmental Management and Agriculture West Pomeranian University of Technology in Szczecin, Szczecin, Poland.

http://orcid.org/0000-0003-2352-7588

E-mail: marta.rokosa@zut.edu.pl. ^*Corresponding author.

DECLARATION OF CONFLICT OF INTERESTS

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

AUTHORS’ CONTRIBUTIONS

The authors contributed equally to the manuscript.

SCIMAGO INSTITUTIONS RANKINGS

Figures | Tables

Figures (3)
Tables (2)

Thumbnail

Figure 1
Stevia growing on MS with 1 mg ∙ dm^-3 BAP (A), without growth regulators (B) and with 5 mg ∙ dm^-3 KIN.

Thumbnail

Figure 2
Stevia growing on MS with 1 mg ∙ dm^-3 IAA (A), 8 mg ∙ dm^-3 IBA (B), 2 mg ∙ dm^-3 IBA (C), without growth regulators (D) and with 8 mg ∙ dm^-3 NAA (E).

Thumbnail

Figure 3
Stevia (A) and its root system (B) growing on MS with 1 mg ∙ dm^-3 NAA and with 4 mg ∙ dm^-3 IAA (C, D).

Thumbnail

Table 1
Mean values of morphological characters of plants propagated on media with variable cytokinin content.

Thumbnail

Table 2
Mean values of morphological characters of plants propagated on media with a variable content of plant growth regulators (rooting).

Figure 1 Stevia growing on MS with 1 mg ∙ dm^-3 BAP (A), without growth regulators (B) and with 5 mg ∙ dm^-3 KIN.

Figure 2 Stevia growing on MS with 1 mg ∙ dm^-3 IAA (A), 8 mg ∙ dm^-3 IBA (B), 2 mg ∙ dm^-3 IBA (C), without growth regulators (D) and with 8 mg ∙ dm^-3 NAA (E).

Figure 3 Stevia (A) and its root system (B) growing on MS with 1 mg ∙ dm^-3 NAA and with 4 mg ∙ dm^-3 IAA (C, D).

Table 1 Mean values of morphological characters of plants propagated on media with variable cytokinin content.

Cytokinin concentration [mg∙dm^-3]		Length of shoots [cm]		No. of shoots		No. of leaves		Fresh weight [g]		No. of roots		Length of roots [cm]
MS		2.80	b^*	1.42	e	9.60	g	0.053	f	0.64	bc	0.29	cd
0.5	BAP	2.78	b	3.04	a	19.04	ab	0.157	ab	0.40	bcd	0.13	de
1	BAP	2.42	c	3.38	a	21.17	a	0.157	a	0.00	e	0.00	e
2		1.90	f	2.63	b	17.08	bc	0.124	cd	0.00	e	0.00	e
5		2.14	de	2.37	b	13.61	d	0.100	de	0.00	e	0.00	e
0.5	2iP	2.96	b	2.17	c	14.88	c	0.167	a	0.00	e	0.00	e
1		2.20	cde	2.69	b	15.39	cd	0.123	cd	0.00	e	0.00	e
2		1.80	f	2.05	cd	12.06	ef	0.100	de	0.00	e	0.00	e
5		1.96	ef	2.33	bc	18.64	b	0.173	a	0.00	e	0.00	e
0.5	KIN	3.65	a	1.79	de	11.46	fg	0.132	bc	1.21	a	0.81	a
1		2.35	cd	1.75	de	10.79	fg	0.076	ef	0.29	cde	0.41	bc
2		2.81	b	2.05	cd	14.09	cde	0.156	ab	0.74	b	0.63	ab
5		2.45	c	2.50	b	15.17	c	0.183	a	0.21	de	0.16	de

*means marked with the same letters do not differ significantly at the significance level 0.05.

Table 2 Mean values of morphological characters of plants propagated on media with a variable content of plant growth regulators (rooting).

Cytokinin concentration [mg∙dm^-3]		Length of shoots [cm]		No. of shoots		No. of leaves		Fresh weight [g]		No. of roots		Length of roots [cm]
MS		4.47^*	ab	1.40	abc	14.24	ab	4.80	abc	2.96	ab	0.38	cd
1	IAA	5.24	a	1.68	a	18.48	a	4.16	abcd	4.00	a	0.59	abcd
2		4.52	ab	1.68	a	16.00	a	3.00	abcde	2.38	abcd	0.51	bcd
4		2.04	cdef	1.40	abc	15.92	a	0.96	de	1.24	cde	0.45	bcd
8		1.30	ef	1.64	a	16.32	a	0.16	e	0.12	e	0.47	bcd
1	IBA	3.80	abc	1.36	abc	15.80	a	6.08	a	3.86	ab	0.78	ab
2		2.27	cde	1.56	ab	14.92	ab	5.48	ab	2.24	bcd	0.88	a
4		0.51	ef	0.68	de	5.24	cd	4.00	abcd	1.20	cde	0.55	abcd
8		0.19	f	0.29	e	3.17	cd	2.71	abcde	0.82	de	0.30	d
1	NAA	3.33	bcd	1.48	abc	14.16	ab	5.76	a	2.70	abc	0.73	abc
2		1.48	def	0.96	bcd	8.72	bc	2.80	abcde	1.18	cde	0.55	abcd
4		0.54	ef	0.88	cde	6.64	cd	1.88	bcde	0.53	e	0.59	abcd
8		0.13	f	0.29	e	1.92	d	1.04	cde	0.16	e	0.38	cd

^*means marked with the same letters do not differ significantly at the significance level 0.05.

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Micropropagation of <i>Stevia rebaudiana</i> plants

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[1] E-mail: marta.rokosa@zut.edu.pl. ^*Corresponding author.