On-line version ISSN 1678-992X
SOUZA, Gustavo Maia; GONCALVES, Antonio Natal and MACHADO NETO, Nelson Barbosa. In vitro growth of Eucalyptus camaldulensis Dehn. progenies under water deficit conditions . Sci. agric. [online]. 1999, vol.56, n.3, pp. 557-562. ISSN 1678-992X. http://dx.doi.org/10.1590/S0103-90161999000300007.
Three Eucalyptus camaldulensis provenances were submitted to water deficit induced in vitro through the addition of 100, 400 and 1000 mM of sorbitol to the growth medium. Shoots were evaluated for growth in height, production of dry matter, moisture content and total proteins in SDS-PAGE (sodium dodecil sulphate-poliacrylamide gel electrophoresis). An inverse relationship was observed between the growth in height, that decreased with the increase of water deficit, and the production of dry matter. There was a general increase in content of total protein in relation to the reduction of the osmotic potential of the growth medium, and the diferenciation of two proteins (36 and 45 kDa).
Keywords : Eucalyptus camaldulensis; water deficit; growth; proteins; tissue culture.