SciELO - Scientific Electronic Library Online

 
vol.59 issue1In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limoniaNitrogen treatment of carnation (Dianthus caryophyllus l., Caryophyllaceae) and in vitro calogenesis author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Scientia Agricola

On-line version ISSN 1678-992X

Abstract

RICCI, Adriana Patrícia; MOURAO FILHO, Francisco de Assis Alves; MENDES, Beatriz Madalena Januzzi  and  PIEDADE, Sonia Maria de Stefano. Somatic embryogenesis in Citrus sinensis, C. reticulata AND C. nobilis x C. deliciosa. Sci. agric. (Piracicaba, Braz.) [online]. 2002, vol.59, n.1, pp.41-46. ISSN 1678-992X.  https://doi.org/10.1590/S0103-90162002000100005.

Most of the plant regeneration processes in citrus, through tissue culture, involve indirect somatic embryogenesis. The optimization of these processes is important for the development of in vitro plant improvement and micropropagation studies. Studies to evaluate the effect of different carbohydrates in somatic embryogenesis were conducted using calli from 'Ponkan' mandarin (Citrus reticulata, Blanco), 'Cravo' mandarin (C. reticulata), 'Itaboraí' sweet orange (C. sinensis L. Osbeck.), 'Valencia' sweet orange (C. sinensis) and 'Kinnow' mandarin (C. nobilis Loureiro x C. deliciosa Tenore). The culture medium used was MT supplemented with sucrose, galactose, glucose, maltose or lactose with the following concentrations of 18, 37, 75, 110, and 150 mM. The culture medium used for the maturation of somatic embryos had 0, 15, 29, 44, 58 and 73 mM of sucrose, in presence or absence of 0.5 g L-1 of activated charcoal. Seventy-three mM of sucrose with 0.1 mg L-1 of GA3 in the presence or absence 0.5 g L-1 of activated charcoal was also tested. Overall, the carbohydrates galactose or lactose induced a higher number of somatic embryos. Sucrose concentrations of 58 and 73 mM generated a higher number of plantlets from mature embryos of 'Ponkan' mandarin and 'Valencia' sweet orange.

Keywords : callus; carbohydrate; citrus; somatic embryo.

        · abstract in Portuguese     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License