On-line version ISSN 1678-992X
BORGES, Aline et al. CTAB methods for DNA extraction of sweetpotato for microsatellite analysis. Sci. agric. (Piracicaba, Braz.) [online]. 2009, vol.66, n.4, pp. 529-534. ISSN 1678-992X. http://dx.doi.org/10.1590/S0103-90162009000400015.
Microsatellite markers have proved to be useful in genetic diversity assessments of sweetpotato (Ipomoea batatas) but practical DNA extraction methods to ensure good quality and quantity DNA for these studies are yet to be established. This study compares the efficiency of three modified methodologies for DNA extraction of six sweetpotato landraces using the CTAB extraction buffer in regard to quantity and purity of DNA quantification and microsatellite band patterns. All methodologies yielded satisfactory results, but the method based in leaf tissue macerated in liquid nitrogen was deemed more adequate because of its simplicity and lower cost. However, the method based in dry leaf tissue was considered more advantageous, first because elicits practicability in the plant acquisition and drying process, especially when the collection is performed in situ, and also because its simplicity makes possible the cold storage of the dry, ground samples for future DNA extractions.
Keywords : Ipomoea batatas; SSR; DNA isolation; landraces; protocol.