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Print version ISSN 0103-9016
ROCHA, Kelly Cristina Gonçales et al. Only the B biotype of Bemisia tabaci is present on vegetables in São Paulo State, Brazil. Sci. agric. (Piracicaba, Braz.) [online]. 2011, vol.68, n.1, pp.120-123. ISSN 0103-9016. http://dx.doi.org/10.1590/S0103-90162011000100018.
Bemisia tabaci (Genn.) is one of the most important pests in cultivated areas of vegetables and ornamental crops around the world. Based on the mitochondrial cytochrome oxidize I (mtCOI) sequence, there is evidence that B. tabaci should be considered a cryptic species complex of 11 groups containing 24 species. Two of the groups, Middle East-Asia Minor 1 and Mediterranean include biotypes B and Q, respectively. In this study we evaluated the mtCOI sequence of B. tabaci populations collected in sites of the state of São Paulo, Brazil. Using PCR-RFLP with Taq I, a typical biotype B profile was obtained for all specimens. Based on the comparison with mtCOI reference sequences we found four haplotypes all belonging to the Middle East-Asia Minor 1. They occurred in the hosts pepper (Capsicum annuum L.), tomato (Solanum lycopersicum L.), eggplant (Solanum melongena L.) and cucurbitaceae plants.
Keywords : RFLP; cytochrome oxidase I gene; sequencing; whiteflies.