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Polímeros

versão impressa ISSN 0104-1428

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ZIMOCH-KORZYCKA, Anna et al. The use of lysozyme to prepare biologically active chitooligomers. Polímeros [online]. 2015, vol.25, n.1, pp.35-41. ISSN 0104-1428.  https://doi.org/10.1590/0104-1428.1630.

Two types of crustacean commercial chitosans (CS1, CS2) were dissolved in lactic acid solutions, hydrolysed by lysozyme and finally fractioned by methanol solutions into two parts containing chito-oligomers (CS-O1, CS-O2). The antioxidant power and antimicrobial properties of both fractions were studied and compared with non-hydrolysed CS1 and CS2. The antioxidant properties were determined by the ferric ion reducing antioxidant power (FRAP) method while the bioactive properties were evaluated against a strain of Listeria monocytogenes. CS-O obtained from the solid fraction of the chito-oligomers solid fractions treated with 90% methanol showed the highest reducing power. Microbiological tests showed that CS-O exhibit higher antilisterial activity than CS.

Palavras-chave : antilisterial activity; antioxidant; chito-oligomers; chitosan; lysozyme.

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