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Revista da Associação Médica Brasileira

Print version ISSN 0104-4230On-line version ISSN 1806-9282

Abstract

MACHADO, Isabela Nelly; CASTILHO, Lilian; PELLEGRINO JR., Jordão  and  BARINI, Ricardo. Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background. Rev. Assoc. Med. Bras. [online]. 2006, vol.52, n.4, pp.232-235. ISSN 0104-4230.  http://dx.doi.org/10.1590/S0104-42302006000400022.

OBJECTIVE: To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. METHODS: We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested for exon 10 and intron 4 gene regions by allele specific-PCR. Fetal RHD genotyping by PCR on maternal plasma was compared to serologic RhD typing in the neonatal period. RESULTS: Samples were obtained as follows: 15 in the 1st, 37 in the 2nd and 29 in the 3rd trimester. General accuracy was 97.3%, sensitivity of 98.3% and specificity of 93.8%. CONCLUSIONS: Conventional PCR is an accurate method for fetal RHD genotyping on maternal plasma, even in a population of mixed ethnic origin.

Keywords : Free fetal DNA; PCR; RHD genotyping; Maternal plasma; Prenatal diagnosis.

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