Abstract - A cultivation strategy based on some previous knowledge of the metabolism of Escherichia coli BL21 (DE3) pLysS containing the troponin C gene cloned into plasmid pET was developed and applied through the use of simple fermentation equipment and a feed-forward control strategy in order to achieve a high cell concentration <FONT FACE="Symbol">¾</font> 92 g l-1 dry cell weight <FONT FACE="Symbol">¾</font> and a high cell productivity <FONT FACE="Symbol">¾</font> 3.7 g l-1 h-1.
Acetic acid; feed-forward; high cell concentration; recombinant Escherichia coli
