Brazilian Journal of Chemical Engineering
On-line version ISSN 0104-6632
MINAMI, N.M.; LUCARINI, A.C. and KILIKIAN, B.V.. Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography. Braz. J. Chem. Eng. [online]. 1999, vol.16, n.2, pp. 193-198. ISSN 0104-6632. http://dx.doi.org/10.1590/S0104-66321999000200012.
In this study, a preparative size-exclusion chromatography of two different clarified media obtained from submerse and semisolid culture of the mold Aspergillus awamori was carried out. Characterization and comparison of the quantities of glucoamylase and contaminant proteins present in these media were possible. Glucoamylase is the protein with the higher molecular weight in both media analyzed, varying from 72 to 80kDa in the submerse culture and from 68 to 90kDa in the semisolid culture. Also, glucoamylase protein concentration is higher in the submerse culture than in the semisolid culture. The other proteins in the submerse culture presented molecular weights lower than 12kDa and in the semisolid culture their molecular weights varied from 21 to 37kDa and below 10kDa.
Keywords : Glucoamylase; gel filtration chromatography; purification; submerse culture; semisolid culture.