SciELO - Scientific Electronic Library Online

 
vol.17 issue4-7Multivariable low order structured-controller design by frequency response approximationPurification of alpha-galactosidase from seeds of Sesbania marginata author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Brazilian Journal of Chemical Engineering

Print version ISSN 0104-6632On-line version ISSN 1678-4383

Abstract

BAPTISTA-NETO, A.; GOUVEIA, E.R.; BADINO-JR, A.C.  and  HOKKA, C.O.. Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus. Braz. J. Chem. Eng. [online]. 2000, vol.17, n.4-7, pp.809-818. ISSN 0104-6632.  http://dx.doi.org/10.1590/S0104-66322000000400043.

The kinetics of clavulanic acid production process by Streptomyces clavuligerus NRRL 3585 was studied. Experiments were carried out in a 4 liters bioreactor, utilizing 2 complex media containing glycerol as the carbon and energy source, and peptone or Samprosoy 90NB (soybean protein) as nitrogen source. Temperature was kept at 28°C and the dissolved oxygen was controlled automatically at 40 % saturation value. Samples were withdrawn for determination of cell mass (only peptone medium), glycerol and product concentrations. Gas analyzers allowed on line determination of CO2 and O2 contents in the exit gas. With Samprosoy, cell mass was evaluated by determining glycerol consumption and considering the cell yield, YX/S, as being the same for both cases. Oxygen uptake and CO2 production rates were strongly related to growth and substrate consumption, allowing determination of stoichiometric constants in relation to growth, substrate, oxygen, product and carbon dioxide.

Keywords : clavulanic acid; Streptomyces clavuligerus; kinetic model.

        · text in English

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License