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Brazilian Journal of Chemical Engineering

Print version ISSN 0104-6632On-line version ISSN 1678-4383


CASTRO, H.F.; SILVA, M.L.C.P.  and  SILVA, G.L.J.P. Evaluation of inorganic matrixes as supports for immobilization of microbial lipase. Braz. J. Chem. Eng. [online]. 2000, vol.17, n.4-7, pp.849-858. ISSN 0104-6632.

Candida rugosa was immobilized by physical adsorption on several inorganic supports using hexane as coupling medium. The enzymatic activities of the different derivatives were determined by both hydrolysis of olive oil and esterification of n-butanol with butyric acid. The results were compared to previous data obtained by using a controlled porous silica matrix. The goal was to contribute in searching inexpensive supports for optimum lipase performance. All supports examined exhibited good properties for binding the enzyme lipase. Zirconium phosphate was the best support, giving the highest percentage of protein fixation (86%) and the highest retention of lipase activity after immobilization (34%). The operational stability performance for niobium oxide derivative was improved by previously activated the support with silane and glutaraldehyde. Thermal stabilities were also examined by thermal gravimetric analysis (TG).

Keywords : Inorganic matrixes; lipase; immobilization; esterification; operational stability; thermal stability.

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