Brazilian Journal of Chemical Engineering
Print version ISSN 0104-6632
IANNUCCI, N.B. et al. Affinity chromatography with pseudobiospecific ligands on high-performance supports for purification of proteins of biotechnological interest. Braz. J. Chem. Eng. [online]. 2003, vol.20, n.1, pp. 27-32. ISSN 0104-6632. http://dx.doi.org/10.1590/S0104-66322003000100006.
High-performance affinity matrices were obtained by attaching pseudobiospecific ligands to hollow-fibre membranes. The neutral protease contained in FlavourzymeTM was purified to homogeneity with Yellow 4R-HE affinity hollow-fibre membranes. Immobilisation of Red HE-3B allowed purification of a milk-clotting enzyme obtained by solid-state culture of Mucor bacilliformis. Copper immobilisation through iminodiacetic acid allowed fractionation of Biocon Bioconcentrated PlusTM to separate the pectinesterase-containing fraction. The productivity of the developed processes - 1900, 94 and 750 U/ml.min, respectively - was 10- to 15-fold higher than that achieved with the same ligands immobilised on agarose-based soft gels, mainly due to the shortening of the purification processes.
Keywords : affinity chromatography; pseudobiospecific ligands; protein purification.