SciELO - Scientific Electronic Library Online

vol.21 número2Expression of Pisum sativum defensin 1 (Psd1) in shaking flasks and bioreactor cultivations of recombinant Pichia pastoris at different pHsEstimation of mass transfer parameters in a Taylor-Couette-Poiseuille heterogeneous reactor índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Brazilian Journal of Chemical Engineering

versión impresa ISSN 0104-6632versión On-line ISSN 1678-4383


GALESI, A. L. L.; TAMASHIRO, W. M. S. C.  y  MORAES, A. M.. The effect of medium composition on interleukin-2 production by murine EL-4 thymoma cells. Braz. J. Chem. Eng. [online]. 2004, vol.21, n.2, pp.165-173. ISSN 0104-6632.

Due to the role of interleukin-2 (IL-2) in the mediation of immune response, this cytokine has been used in the treatment of some types of cancer and infectious diseases. However, relatively high levels of this cytokine are required to achieve significant activity. The aim of this work was to study a culture medium composition designed to increase the production of IL-2 by suspended murine EL-4 cells. The cultivations were carried out aiming at producing IL-2 in stirred bioreactors. The effects of concentration of glutamine, phorbol-12-myristate-13-acetate (PMA), concanavalin A (Con A), Pluronic F68, and fetal calf serum (FCS) on cell viability and IL-2 production were evaluated. PMA alone was more efficient in IL-2 production than it was in association with Con A. The maximum IL-2 production was around 162 ng/mL with 856 ng/mL PMA and 1.45% (v/v) FCS.

Palabras clave : cytokine; bioreactors; interleukin-2.

        · texto en Inglés     · Inglés ( pdf epdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons