Brazilian Journal of Chemical Engineering
On-line version ISSN 0104-6632
CASTRO, B. D. and AZNAR, M.. Liquid-liquid equilibrium of water + PEG 8000 + magnesium sulfate or sodium sulfate aqueous two-phase systems at 35°C: experimental determination and thermodynamic modeling. Braz. J. Chem. Eng. [online]. 2005, vol.22, n.3, pp. 463-470. ISSN 0104-6632. http://dx.doi.org/10.1590/S0104-66322005000300014.
Liquid-liquid extraction using aqueous two-phase systems is a highly efficient technique for separation and purification of biomolecules due to the mild properties of both liquid phases. Reliable data on the phase behavior of these systems are essential for the design and operation of new separation processes; several authors reported phase diagrams for polymer-polymer systems, but data on polymer-salt systems are still relatively scarce. In this work, experimental liquid-liquid equilibrium data on water + polyethylene glycol 8000 + magnesium sulfate and water + polyethylene glycol 8000 + sodium sulfate aqueous two-phase systems were obtained at 35°C. Both equilibrium phases were analyzed by lyophilization and ashing. Experimental results were correlated with a mass-fraction-based NRTL activity coefficient model. New interaction parameters were estimated with the Simplex method. The mean deviations between the experimental and calculated compositions in both equilibrium phases is about 2%.
Keywords : Liquid-liquid equilibrium; Aqueous two-phase systems; Data; poly(ethylene glycol); Salt; NRTL model.