Resumo

NETO, A. B. et al. A study on clavulanic acid production by Streptomyces clavuligerus in batch, fed-batch and continuous processes. Braz. J. Chem. Eng. [online]. 2005, vol.22, n.4, pp. 557-563. ISSN 0104-6632.  http://dx.doi.org/10.1590/S0104-66322005000400008.

Clavulanic acid (CA) is a potent inhibitor of b-lactamases, enzymes that are responsible for the hydrolysis of b-lactam antibiotics. It is a secondary metabolite produced by the filamentous aerobic bacterium Streptomyces clavuligerus in submerged cultivations. In the present work clavulanic acid production in batch, fed-batch and continuous bioreactors was studied with the objective of increasing productivity. The operating conditions: temperature, aeration and agitation, were the same in all cases, 28º C, 0.5 vvm and 800 rpm, respectively. The CA concentration obtained in the fed-batch culture, 404 mg L^-1, was ca twice the value obtained in the batch culture, 194 mg L^-1, while 293 mg L^-1 was obtained in the continuous culture. The highest productivity was obtained in the continuous cultivation, 10.6 mg L^-1 h^-1, as compared with 8.8 mg L^-1 h^-1 in the fed-batch process and 3.5 mg L^-1 h^-1 in the batch process, suggesting that continuous culture of Streptomyces clavuligerus is a promising strategy for clavulanic acid production.

Palavras-chave : Clavulanic acid; Bioreactors; Enzymes.