Brazilian Journal of Chemical Engineering
versão impressa ISSN 0104-6632
SOUZA, F. A. S. D.; SALGUEIRO, A. A. e ALBUQUERQUE, C. D. C.. Production of bioemulsifiers by Yarrowia lipolytica in sea water using diesel oil as the carbon source. Braz. J. Chem. Eng. [online]. 2012, vol.29, n.1, pp. 61-67. ISSN 0104-6632. http://dx.doi.org/10.1590/S0104-66322012000100007.
The objective of this work was to investigate, on a flask scale, the production of bioemulsifiers by Yarrowia lipolytica in the presence of sea water, supplemented with nitrogen and phosphate sources, using diesel oil as substrate. A full 24 factorial design was conducted to investigate the effects and interactions of the nutrient concentrations (diesel oil, urea, ammonium sulfate and monobasic potassium phosphate) on the response variables: emulsification activity and surface tension of the cell-free cultures. High emulsification activities (> 5,4 UEA) were determined after 168 h in all the experiments. The interactions among diesel oil, urea and monobasic potassium phosphate favored the emulsification with statistical significance. A correlation between the increase of emulsification activity and the reduction of surface tension was not identified.
Palavras-chave : Biosurfactant; Bioemulsifier; Diesel oil; Sea water; Yarrowia lipolytica; Factorial design.