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vol.34 número1BIOMASS PYROLYSIS KINETICS: A REVIEW OF MOLECULAR-SCALE MODELING CONTRIBUTIONSSYNTHESIS, CHARACTERIZATION AND APPLICATION OF A POLYURETHANE-BASED SUPPORT FOR IMMOBILIZING MEMBRANE-BOUND LIPASE índice de autoresíndice de assuntospesquisa de artigos
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Brazilian Journal of Chemical Engineering

versão impressa ISSN 0104-6632versão On-line ISSN 1678-4383

Resumo

ANSCHAU, A.; CARUSO, C. S.; KUHN, R. C.  e  FRANCO, T. T.. VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS. Braz. J. Chem. Eng. [online]. 2017, vol.34, n.1, pp.19-27. ISSN 0104-6632.  http://dx.doi.org/10.1590/0104-6632.20170341s20140222.

A colorimetric sulfo-phospho-vanillin (SPV) method was used to validate a high throughput method for total lipids analysis in fresh and lyophilized oleaginous microorganisms. This method uses a small amount of biological material, does not require a lot of sample manipulation, can be automated, is reproducible and easy to implement. A 96-well microplate SPV assay allows one to determine quickly total lipids in lyophilized cells of oleaginous yeast and microalgae. The new assay method possesses many advantages compared to the others described in the literature: requires a small amount sample, less time (around 1 h) and less labor and does not require organic reagents like chloroform in the reaction.

Palavras-chave : Colorimetric method; Microplate; Oleaginous yeast and microalgae; Lipid.

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