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vol.34 número1EVALUATION OF THE FLOCCULATION EFFICIENCY OF Chlorella vulgaris MEDIATED BY Moringa oleifera SEED UNDER DIFFERENT FORMS: FLOUR, SEED CAKE AND EXTRACTS OF FLOUR AND CAKESONO-OXIDATIVE PRE-TREATMENT OF WASTE ACTIVATED SLUDGE BEFORE ANAEROBIC BIODEGRADATION índice de autoresíndice de assuntospesquisa de artigos
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Brazilian Journal of Chemical Engineering

versão impressa ISSN 0104-6632versão On-line ISSN 1678-4383

Resumo

MOHANTY, Satya Sundar  e  JENA, Hara Mohan. BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11. Braz. J. Chem. Eng. [online]. 2017, vol.34, n.1, pp.75-84. ISSN 0104-6632.  http://dx.doi.org/10.1590/0104-6632.20170341s20150388.

In the present study, a pure culture of bacterium (Pseudomonas sp. Strain NBM11) was isolated from the soil sample from a site contaminated with medical wastes and wastewater. The isolated strain can degrade up to 1000 mg/L of phenol completely. It was observed that temperature, pH and initial concentration of phenol play key roles in determining the rate of phenol degradation. The isolated strain exhibited the maximal degradation of the substrate within a range of pH 6.8 to 7.2 and an incubation temperature between 30 ºC and 32 ºC. It was found that by increasing the concentration of phenol, the lag phase gets extended due to the inhibitory nature of phenol. The kinetic parameters such as µmax (maximum specific growth rate), Ks (half-saturation coefficient) and Ki (substrate inhibition constant) were estimated as 0.184 1/h, 7.79 mg/L and 319.24 mg/L, respectively, by fitting the growth kinetics data to the Haldane model of substrate inhibition. The bacterial strain was immobilized in alginate beads and its phenol degradation efficiency was observed to increase many fold. The immobilized cells were found to be used efficiently for seven cycles consecutively without any decrease in their efficiency.

Palavras-chave : Pseudomonas sp.; Immobilization; Calcium alginate; Acinetobacter calcoaceticus; Phenol Biodegradation.

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