Anais da Sociedade Entomológica do Brasil
On-line version ISSN 1981-5328
BOGO, Amauri and MANTLE, Peter. Oligosaccharides in the honeydew of Coccoidea scale insects: Coccus hesperidum L. and a new Stigmacoccus sp. in Brazil. An. Soc. Entomol. Bras. [online]. 2000, vol.29, n.3, pp. 589-595. ISSN 1981-5328. http://dx.doi.org/10.1590/S0301-80592000000300022.
Analysis of the honeydew from an as yet undescribed, though distinctive, Brazilian Stigmacoccus sp. (near S. asper Hempel) by paper chromatography, Fast atom bombardment (FAB-MS) and Gas chromatography-mass spectrometry (GC-MS) identified fructose and glucose as monosaccharides and sucrose, maltose, trehalulose, trehalose and a hexose-hexitol as disaccharides. Erlose and glucosyl erlose have been identified as the tri- and tetra-saccharides in Stigmacoccus sp. and characterised for the first time in scale insects by modern techniques of linkage analysis. The same erlose oligosaccharides were recognised in honeydew of the common scale insect Coccus hesperidum L., together with the pentamer of this series, maltosyl erlose, therefore recognising that specific metabolic transformations of sugars into this oligomeric series occur rather widely in Coccoidea scale insects.
Keywords : Insecta; FAB-MS; GC-MS; erlose; maltose; metabolic transformation.