Anais Brasileiros de Dermatologia
On-line version ISSN 1806-4841
SANCHEZ, Ana Paula Galli et al. Study on lymphocyte proliferation in nickel sensitive patients. An. Bras. Dermatol. [online]. 2005, vol.80, n.2, pp. 149-158. ISSN 1806-4841. http://dx.doi.org/10.1590/S0365-05962005000200005.
BACKGROUND: The diagnosis of nickel sensitivity is made by epicutaneous patch testing. OBJECTIVE: To develop a more sensitive and specific test. PATIENTS AND METHODS: Nineteen patients with positive patch test reactions to nickel and 25 controls were submitted to lymphocyte proliferation test. Mononuclear cells were isolated from peripheral venous blood and cultivated in triplicate in culture plates (2x105 cells/well) with: culture medium only, nickel sulfate (156.25; 78.13; 19.53; 9.77 and 2.44µM) and optimal concentrations of Candida albicans antigen as well as pokeweed, phytohemagglutinin A and anti-CD3 antibody (OKT3) mitogens. Tritiated thymidine was added to plates, radioactivity incorporated by cells was measured and the results expressed by the stimulation index (SI). RESULTS: The lymphocyte proliferative response was higher in cases than in controls in all nickel concentrations tested. Considering positive test reactions when SI > 3, none of the controls and 16 (84.21%) cases were positive in at least one of five concentrations used. The proliferative responses to Candida albicans and mitogens were similar in cases and controls, demonstrating normal cellular immunity in both groups. CONCLUSION: The lymphocyte proliferation test is useful in diagnosis of nickel sensitivity.
Keywords : Lymphocyte activation; Dermatitis, contact; Nickel.