SciELO - Scientific Electronic Library Online

vol.81 issue5Thoracic sympathetic block by clamping for treatment of hyperhidrosisIs incisional biopsy helpful in the histopathological classification of basal cell carcinoma? author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Anais Brasileiros de Dermatologia

Print version ISSN 0365-0596On-line version ISSN 1806-4841


HSIEH, Ricardo et al. Genetic analysis of p16 gene by PCR-SSCP technique and protein p16 expression in oral mucosa and skin melanomas. An. Bras. Dermatol. [online]. 2006, vol.81, n.5, pp.433-441. ISSN 1806-4841.

BACKGROUND: Deletion and mutation of gene CDKN2a, which encodes a specific inhibitor of cyclin-dependent kinase 4 (CDK4), the protein p16, has been regarded as related to cutaneous melanoma tumorigenesis. However, little is known about those alterations in oral mucosa melanomas. OBJECTIVES: To verify possible p16 gene mutations and its protein expression in sporadic melanomas in oral mucosa and skin. MATERIALS AND METHODS: 36 primary sporadic melanoma paraffin-embedded specimens (seven oral mucosa and 29 skin lesions) were subjected to molecular analysis of exons 1, 2 and 3 of p16 gene using polymerase chain reaction/single strand conformational polymorphism technique. p16 protein expression was demonstrated by an immunohistochemical technique. Data obtained were correlated with tumor thickness. RESULTS: Five out of seven oral melanomas, and 17 out of 29 skin lesions displayed signs of alteration in p16 gene molecular analysis. Alterations in exon 2 of p16 gene were the most frequent. Protein p16 expression was observed in only one oral melanoma and in 10/13 (76.9%) skin melanomas up to 1.0 mm-thick and in 7/8 (87.5%) lesions thicker than 1.0 mm. CONCLUSIONS: Frequency of alterations disclosed by p16 gene molecular analysis in oral mucosa melanomas was 71.42% and 58.6% in cutaneous lesions. The obtained data suggest that p16 gene alterations play a role in the pathogenesis of sporadic melanoma of the oral mucosa. Neither protein p16 expression, nor p16 gene alteration had correlation with tumor thickness.

Keywords : Genes, p16; Immunohistochemistry; Melanoma; Molecular biology.

        · abstract in Portuguese     · text in English | Portuguese     · English ( pdf ) | Portuguese ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License