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Anais Brasileiros de Dermatologia
On-line version ISSN 1806-4841
DUARTE, Maria Luisa and ROCHAEL, Mayra Carrijo. Histopathological and immunohistochemical profile of the American cutaneous leishmaniasis with emphasis on FXIIIa+ dermal dendrocytes. An. Bras. Dermatol. [online]. 2006, vol.81, n.6, pp.541-548. ISSN 1806-4841. http://dx.doi.org/10.1590/S0365-05962006000600005.
BACKGROUND: Mucocutaneous leishmaniasis is a parasitic infectious disease with relevant immunological aspects. OBJECTIVE: To study the histopathological and immunohistochemical aspects of 21 leishmaniasis tegumentary biopsies. METHODS: Polyclonal anti-Leishmania antibody was used to confirm the presence of Leishmania amastigotes. Histopathological classification comprised I-V standard groups. The immunopathological pattern was evaluated as to the presence of FXIIIa+ dermal dendrocytes, CD1a+ Langerhans cells, CD68+ macrophages, CD20+ B lymphocytes, and CD3+ T lymphocytes. The FXIIIa+ cells were quantified and compared to specimens of normal skin obtained from unexposed areas. The other cells were counted in a semi-quantitative way. The number of FXIIIa+ cells was statistically analyzed using the Mann-Whitney test. RESULTS: Among the histopathological groups, I and II standards prevailed. The FXIIIa+ cells were observed for different aspects and compared to normal skin, without significant statistical differences (p = 0.157). There was no relation between the amount of CD1a+, CD68+, CD20+ and CD3+ cells when compared to each other or to FXIIIa+ cells. CONCLUSION: There was no difference between the number of FXIIIa+ cells in leishmaniasis and in normal skin. However, more studies are needed in order to understand the role of FXIIIa+ cells in leishmaniasis.
Keywords : Autoimmunity; Cutaneous leishmaniasis; Dendritic cells; Factor XIIIa.